Activities of fluconazole and voriconazole against 1,586 recent clinical isolates of Candida species determined by Broth microdilution, disk diffusion, and Etest methods: report from the ARTEMIS Global Antifungal Susceptibility Program, 2001

M A Pfaller; D J Diekema; S A Messer; L Boyken; R J Hollis

doi:10.1128/JCM.41.4.1440-1446.2003

Back

Activities of fluconazole and voriconazole against 1,586 recent clinical isolates of Candida species determined by Broth microdilution, disk diffusion, and Etest methods: report from the ARTEMIS Global Antifungal Susceptibility Program, 2001

Journal article

Open access

Peer reviewed

Activities of fluconazole and voriconazole against 1,586 recent clinical isolates of Candida species determined by Broth microdilution, disk diffusion, and Etest methods: report from the ARTEMIS Global Antifungal Susceptibility Program, 2001

M A Pfaller, D J Diekema, S A Messer, L Boyken and R J Hollis

Journal of clinical microbiology, Vol.41(4), pp.1440-1446

04/2003

DOI: 10.1128/JCM.41.4.1440-1446.2003

PMCID: PMC153922

PMID: 12682127

Files and links (1)

url

https://doi.org/10.1128/JCM.41.4.1440-1446.2003View

Published (Version of record) Open Access

Abstract

The ARTEMIS Global Antifungal Susceptibility Program (ARTEMIS Program) was initiated in 2001 to provide focused surveillance of the activities of fluconazole and voriconazole against Candida spp. isolated from blood and other normally sterile sites. A total of 1,586 episodes of infection were detected at 61 international study sites. Overall, 57.7% of the infections were due to Candida albicans, followed by C. glabrata (14.8%), C. parapsilosis (12.5%), C. tropicalis (9.4%), C. krusei (2.7%), and C. lusitaniae (1.5%). Isolates of C. albicans, C. parapsilosis, and C. tropicalis were all highly susceptible to fluconazole (for 99% of the isolates the MICs were <or=8 microg/ml). Likewise, 99 to 100% of these species were inhibited by <or=1 microg of voriconazole per ml. Voriconazole was also active against C. glabrata (93% of the isolates were susceptible [MICs <or= 1 microg/ml]) and C. krusei (100% of the isolates were susceptible). The agar-based Etest and disk diffusion methods performed well for the testing of both fluconazole and voriconazole compared to the broth microdilution MIC reference method. These observations establish the continued importance of C. albicans as a pathogen and the sustained activity of fluconazole and the broad spectrum of activity of voriconazole and will serve as the first-year benchmark for the ARTEMIS Program. Continued surveillance and refinement of broth- and agar-based test methods will help to identify susceptibility trends and improve the laboratory capability for antifungal susceptibility testing.

Antifungal Agents - pharmacology

Fluconazole - pharmacology

Humans

Pyrimidines - pharmacology

Candidiasis - microbiology

Candida - isolation & purification

Triazoles - pharmacology

Candida - classification

Voriconazole

Candida - drug effects

Drug Resistance, Fungal

Microbial Sensitivity Tests - methods

Population Surveillance

Details

Title: Subtitle: Activities of fluconazole and voriconazole against 1,586 recent clinical isolates of Candida species determined by Broth microdilution, disk diffusion, and Etest methods: report from the ARTEMIS Global Antifungal Susceptibility Program, 2001
Creators: M A Pfaller - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine and College of Public Health, University of Iowa, Iowa City, Iowa 52242, USA. michael-pfaller@uiowa.edu
D J Diekema
S A Messer
L Boyken
R J Hollis
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.41(4), pp.1440-1446
DOI: 10.1128/JCM.41.4.1440-1446.2003
PMID: 12682127
PMCID: PMC153922
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 04/2003
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986363802771

Metrics

39 Record Views

147 Times Cited - Web of Science