Acute and subchronic oral toxicities of Pu-erh black tea extract in Sprague–Dawley rats

Di Wang; Kunlong Xu; Ying Zhong; Xiao Luo; Rong Xiao; Yan Hou; Wei Bao; Wei Yang; Hong Yan; Ping Yao; Liegang Liu

doi:10.1016/j.jep.2010.11.068

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Acute and subchronic oral toxicities of Pu-erh black tea extract in Sprague–Dawley rats

Journal article

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Acute and subchronic oral toxicities of Pu-erh black tea extract in Sprague–Dawley rats

Di Wang, Kunlong Xu, Ying Zhong, Xiao Luo, Rong Xiao, Yan Hou, Wei Bao, Wei Yang, Hong Yan, Ping Yao, …

Journal of ethnopharmacology, Vol.134(1), pp.156-164

03/08/2011

DOI: 10.1016/j.jep.2010.11.068

PMID: 21134434

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Abstract

Pu-erh black tea, which is obtained by first parching crude green tea leaves and then undergoes secondary fermentation with microorganisms, has been believed to be beneficial beverages for health for nearly 2000 years in China, Japan and Taiwan area. The present study suggested that high dose (5000mg/kg/day) of BTE intake would be well-tolerated for long-term use as a dietary supplement whatever by animals or human. Pu-erh black tea, which is obtained by first parching crude green tea leaves and then undergoes secondary fermentation with microorganisms, has been believed to be beneficial beverages for health for nearly 2000 years in China, Japan and Taiwan area. But its potential toxicity when administered at a high dose as concentrated extracts has not been completely investigated. The present study was aimed at evaluating potential toxicity of Pu-erh black tea extracts (BTE) from acute and sub-chronic administration to male and female Sprague–Dawley (SD) rats. A single BTE dose of 10,000mg/kg of body weight was administered by oral gavage for acute toxicity in SD rats. Four groups (10 males and 10 females per group) of dose levels of 1250, 2500, and 5000mg/kg/day of the test article, as well as controls (distilled water) were tested as the subchronic toxicity study. No deaths and signs of toxicity occurred during the 14 days of the study. There were no test article related mortalities, body weight gain, feed consumption, clinical observation, organ weight changes, gross finding, clinical or histopathological alterations during the 91-day administration. The LD50 of BTE can be defined as more than 10,000mg/kg, and a dose of 5000mg/kg/day was identified as the no-observed-adverse-effect-level (NOAEL) in this study.

LD50

Pu-erh black tea extracts

Subchronic toxicity

NOAEL

Acute toxicity

Details

Title: Subtitle: Acute and subchronic oral toxicities of Pu-erh black tea extract in Sprague–Dawley rats
Creators: Di Wang - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, PR China
Kunlong Xu - College of Animal Science and Technology, Yunnan Agriculture University, Kunming, Yunnan 650201, PR China
Ying Zhong - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, PR China
Xiao Luo - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, PR China
Rong Xiao - College of Food Science, Yunnan Agriculture University, Kunming, Yunnan 650201, PR China
Yan Hou - Certificate Assessment Center of Yunnan Pu-erh Tea, Yunnan Agriculture University, Kunming, Yunnan 650201, PR China
Wei Bao - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, PR China
Wei Yang - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, PR China
Hong Yan - MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, PR China
Ping Yao - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, PR China
Liegang Liu - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, PR China
Resource Type: Journal article
Publication Details: Journal of ethnopharmacology, Vol.134(1), pp.156-164
DOI: 10.1016/j.jep.2010.11.068
PMID: 21134434
NLM abbreviation: J Ethnopharmacol
ISSN: 0378-8741
eISSN: 1872-7573
Publisher: Elsevier Ireland Ltd
Grant note: name: National Key Technology R&D Program of the People's Republic of China, award: 2007BAD58B05
Language: English
Date published: 03/08/2011
Academic Unit: Epidemiology; Fraternal Order of Eagles Diabetes Research Center
Record Identifier: 9983995170202771

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