Journal article
Acute deletion of central 5‐HT neurons in DTR adult mice decreases body temperature in males more than females
The FASEB journal, Vol.25, pp.854.5-854.5
04/2011
DOI: 10.1096/fasebj.25.1_supplement.854.5
Abstract
Lmx1bf/f/p mice, which lack 5‐HT neurons, have respiratory abnormalities in the perinatal period. However, breathe normally at rest, but fail to thermoregulate in adulthood. We measured body temperature (BT) and ventilation (VE) after acutely deleting 5‐HT neurons in adult mice. We injected diphtheria toxin (DT) in mice that expressed the DT receptor selectively in 5‐HT neurons (Pet1‐Cre/Floxed DTR mice). We measured baseline VE, response to CO2 and BT by plethysmography at 30˚C in adult mice. The number of tryptophan hydroxylase (TpH) positive neurons was then determined.
After 4 injections of 1000 ng DT (once every 10 days), the BT of DTR mice decreased severely when ambient temperature (TA) was 22˚C, from 37 ± 0.4˚C to 33 ± 0.7˚C (p<0.01, t test), but was not different in WT mice. VE and the response to CO2 weren't affected in either group. After higher doses of DT, males decreased BT faster than females (p < 0.01 t‐test). When TA was changed from 36˚C to 30˚C, DTR and WT mice both decreased their BT in 2.5 hr, but lower in males (29.7 ± 0.3˚C) than in females (33.0 ± 1.3˚C:p < 0.01 ANOVA). There were fewer TpH positive neurons in the brainstem in DTR mice than WT mice (2.5 ± 0.7 and 9.1 ± 0.9 for DTR and WT mice, respectively; p < 0.01, ANOVA).
In conclusion, 5‐HT neurons are important for maintenance of body temperature more in males than females. However, DT has effects on WT mice that can confound results with this genetic approach.
Details
- Title: Subtitle
- Acute deletion of central 5‐HT neurons in DTR adult mice decreases body temperature in males more than females
- Creators
- Veronica E Cerpa - Yale UniversityAmalia Gonzalez - Yale UniversityGeorge B Richerson - Yale University
- Resource Type
- Journal article
- Publication Details
- The FASEB journal, Vol.25, pp.854.5-854.5
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.25.1_supplement.854.5
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 04/2011
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Neurosurgery; Neurology
- Record Identifier
- 9984072081702771
Metrics
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