Journal article
Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs
Journal of Toxicology and Environmental Health, Part A, Vol.70(22), pp.1923-1928
01/01/2007
DOI: 10.1080/15287390701551266
PMID: 17966063
Abstract
The acute effects of pure inhaled glucan on respiratory inflammation remain inconclusive and not sufficiently examined with regards to the simultaneous interaction of glucan, endotoxin (LPS) and house dust in airway inflammation. This study aims at determining effects of simultaneous exposure to office dust and glucan on nasal and pulmonary inflammation. This is relevant for humans with occupational exposure in waste handling and farming and buildings with mold problems. Office dust collected from Danish offices was spiked with 1% (1-3)-β-glucan (curdlan). Guinea pig nasal cavity volume was measured by acoustic rhinometry (AR) and animals were exposed by inhalation for 4 hr to curdlan spiked dust, unspiked dust, purified air (negative controls) or LPS (positive controls). After exposure (+5 hr) or the following day (+18 hr) measurements were repeated by AR and followed by bronchoalveolar lavage (BAL). Total and differential cell counts, IL-8 in BAL fluid and change in nasal volume was compared between groups. A 5-10% increase in nasal volume was seen for all groups including clean air except for a significant 5% decrease for spiked-dust inhalation (+18 hr). No marked differences were observed in BAL cells or IL-8 except in LPS-exposed controls. The delayed decrease of nasal cavity volume after exposure to glucan spiked dust suggests a slow effect on the upper airways for curdlan and office dust together, though no pulmonary response or direct signs of inflammation were observed. Glucan spiked office dust exposures produced a delayed nasal sub-acute congestion in guinea pigs compared to office dust alone but extrapolated to nasal congestion in humans, paralleling the nasal congestion seen in human volunteers exposed to the same dust, it may not have clinical importance.
Details
- Title: Subtitle
- Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs
- Creators
- S P StraszekA Adamcakova-DoddNervana MetwaliO F PedersenT SigsgaardP S Thorne
- Resource Type
- Journal article
- Publication Details
- Journal of Toxicology and Environmental Health, Part A, Vol.70(22), pp.1923-1928
- DOI
- 10.1080/15287390701551266
- PMID
- 17966063
- ISSN
- 1528-7394
- eISSN
- 1087-2620
- Language
- English
- Date published
- 01/01/2007
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics
- Record Identifier
- 9983997441002771
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