Journal article
Acute in vivo pulmonary toxicity assessment of occupationally relevant particulate matter from a cellulose nanofiber board
NanoImpact, Vol.17, p.100210
01/2020
DOI: 10.1016/j.impact.2020.100210
PMID: 32968699
Abstract
Cellulose nanofibers (CNFs) are an emerging engineered nanomaterial that are utilized in a variety of applications, including as a replacement for urea-formaldehyde, and other adhesives, as the binding agent in manufactured fiber and particle boards. To ensure the health and well-being of those producing, installing, or otherwise using cellulose nanofiber boards (CNFBs) it is imperative that the particulate matter (PM) produced during CNFB manipulation be evaluated for toxicity. We developed and internally verified a generation system to examine the PM produced by sanding CNFB using aluminum oxide sandpaper. With 80-grit sandpaper our system produced a low dispersity aerosol, as determined by a scanning mobility particle sizer and an optical particle counter, with a geometric mean of 28nm (GSD=1.60). ICP-MS evaluation showed little difference in metal concentrations between CNFB PM and nonsanded CNFB stock. We then used the system to simultaneously generate and expose both male and female C57BL/6J mice acutely for 4h at a concentration of 7.9mg/m3. Sham-exposed controls were treated similarly but without sanding the CNFB. Analysis of bronchoalveolar lavage (BAL) fluid biomarkers showed no signs of inflammatory response at either 4- or 24-hours post-exposure. Further, BAL cell viability, number of total cells, and pulmonary cellular recruitment were not significantly changed between the sham-exposed controls and CNFB-exposed mice. Histology further confirmed no pulmonary toxicity as a result of CNFB PM inhalation. We conclude that inhalation of a high concentration of the PM from manipulation of a CNFB did not produce acute toxic responses within 24h of exposure.
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•A new cellulose nanofiber board (CNFB) procured for toxicological evaluation.•We successfully built and internally verified a system for aerosol generation from CNFB.•Generated aerosol from CNFB was tested and found to be of low acute toxicity in vivo.
Details
- Title: Subtitle
- Acute in vivo pulmonary toxicity assessment of occupationally relevant particulate matter from a cellulose nanofiber board
- Creators
- Nathanial J Parizek - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, Iowa City, IA 52246, USABenjamin R Steines - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USAEzazul Haque - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, Iowa City, IA 52246, USARalph Altmaier - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USAAndrea Adamcakova-Dodd - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USAPatrick T O'Shaughnessy - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USAPeter S Thorne - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, Iowa City, IA 52246, USA
- Resource Type
- Journal article
- Publication Details
- NanoImpact, Vol.17, p.100210
- DOI
- 10.1016/j.impact.2020.100210
- PMID
- 32968699
- NLM abbreviation
- NanoImpact
- ISSN
- 2452-0748
- eISSN
- 2452-0748
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: U01ES027252; DOI: 10.13039/100000002, name: NIH, award: P30 ES005605; DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: NIH U01 ES027252, NIH P30 ES005605
- Language
- English
- Date published
- 01/2020
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics
- Record Identifier
- 9984000926102771
Metrics
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