Journal article
Acute intrauterine infection results in an imbalance between pro- and anti-inflammatory cytokines in the pregnant rabbit.
American journal of reproductive immunology (New York, N.Y. : 1989), Vol.43(5), pp.305-311
05/01/2000
DOI: 10.1111/j.8755-8920.2000.430510.x
PMID: 10872611
Abstract
<p>PROBLEM: Intrauterine infection results in an increase in cytokines. This study compared the time courses for the pro- and anti-inflammatory cytokine responses in 33 pregnant rabbits at 70% gestation. Pro-inflammatory markers were activated nuclear factor-kappa B (NF-kappaB) in placenta and tumor necrosis factor-alpha (TNF-alpha) in amniotic fluid. These were compared to the anti-inflammatory cytokine, interleukin-1 receptor antagonist (IL-1ra), in placenta and uterus.</p>
<p>METHOD OF STUDY: Does were endoscopically inoculated with Escherichia coli through their cervices and sacrificed at six intervals between 0 and 30 hr post-inoculation.</p>
<p>RESULTS: Activated NF-kappaB, determined by electromobility gel shift assay, increased significantly 16 hr after bacterial inoculation (P < or = 0.05). This was directly mirrored by TNF-alpha concentrations, determined by bioassay, in the amniotic fluid. However, IL-1ra levels, determined by enzyme-linked immunosorbent assay, did not increase in response to infection.</p>
<p>CONCLUSIONS: Intrauterine infection results in an imbalance between pro- and anti-inflammatory cytokines that may potentiate infection-induced preterm delivery.</p>
Details
- Title: Subtitle
- Acute intrauterine infection results in an imbalance between pro- and anti-inflammatory cytokines in the pregnant rabbit.
- Creators
- Kimberly K. Leslie - University of IowaScott L. LeeSusan M. WoodcockJill K. DaviesJoan L. EskensRonald S. GibbsEmmet HirschMichael P. ShermanRobert S. McDuffie Jr.
- Resource Type
- Journal article
- Publication Details
- American journal of reproductive immunology (New York, N.Y. : 1989), Vol.43(5), pp.305-311
- DOI
- 10.1111/j.8755-8920.2000.430510.x
- PMID
- 10872611
- NLM abbreviation
- Am J Reprod Immunol
- ISSN
- 1046-7408
- Language
- English
- Date published
- 05/01/2000
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983557560502771
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