Journal article
Acute stretch promotes endothelial cell proliferation in wounded healing mouse skin
Archives of Dermatological Research, Vol.300(9), pp.495-504
10/2008
DOI: 10.1007/s00403-008-0836-3
PMID: 18330587
Abstract
We have developed a novel in vivo model utilizing acute stretch to investigate endothelial cell proliferation as a marker of vascular growth in healing mouse skin. This study is a follow-up to ones revealing immediate stretch improves blood flow, decreases total tissue necrosis, and induces tissue insulin transcription. Dorsal distally based flaps of skin were stretched for 3 min using linear (skin hook) plus hemispherical load cycling (inflated subcutaneous silicone catheter). Unstretched, wounded skin along the back and sternum served as postoperative controls. Laser Doppler flowmetry demonstrated a threefold increase in flap perfusion at postoperative day 7. A stretch-induced sixfold increase in endothelial cell mitogenesis accompanied enhancements in blood flow and extracorporal wound healing over the sternum. Western blots revealed up-regulation/activation of insulin and mitogenic signaling intermediates in stretched skin. Activated insulin and insulin growth factor receptors (pIR/pIGFR), protein kinase B (Akt, pAkt), vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (flk-1) were among the identified stretch-responsive intermediates. These results indicate the benefits of acute stretch are mediated through enhanced vascularity as evidenced by endothelial cell mitogenesis and up-regulation/activation of insulin and key angiogenic effectors in dorsal distally based skin flaps.
Details
- Title: Subtitle
- Acute stretch promotes endothelial cell proliferation in wounded healing mouse skin
- Creators
- Carl Shrader - West Virginia UniversityHolly Ressetar - West Virginia UniversityJia Luo - West Virginia UniversityEugene Cilento - West Virginia UniversityFrank Reilly - West Virginia University
- Resource Type
- Journal article
- Publication Details
- Archives of Dermatological Research, Vol.300(9), pp.495-504
- Publisher
- Springer-Verlag
- DOI
- 10.1007/s00403-008-0836-3
- PMID
- 18330587
- ISSN
- 0340-3696
- eISSN
- 1432-069X
- Language
- English
- Date published
- 10/2008
- Academic Unit
- Pathology
- Record Identifier
- 9984186656002771
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