Acute toxicity of 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) in male Sprague–Dawley rats: Effects on hepatic oxidative stress, glutathione and metals status

Ian Lai; Yingtao Chai; Don Simmons; Gregor Luthe; Mitchell C Coleman; Douglas Spitz; Wanda M Haschek; Gabriele Ludewig; Larry W Robertson

doi:10.1016/j.envint.2009.11.002

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Acute toxicity of 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) in male Sprague–Dawley rats: Effects on hepatic oxidative stress, glutathione and metals status

Journal article

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Acute toxicity of 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) in male Sprague–Dawley rats: Effects on hepatic oxidative stress, glutathione and metals status

Ian Lai, Yingtao Chai, Don Simmons, Gregor Luthe, Mitchell C Coleman, Douglas Spitz, Wanda M Haschek, Gabriele Ludewig and Larry W Robertson

Environment international, Vol.36(8), pp.918-923

2010

DOI: 10.1016/j.envint.2009.11.002

PMCID: PMC2891598

PMID: 19969354

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https://doaj.org/article/937358b79f004facb45cc1f88500943dView

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Abstract

Although polychlorinated biphenyl (PCBs) production, and new uses for PCBs, was halted in the 1970s in the United States, PCBs continue to be used in closed systems and persist in the environment, accumulating in fatty tissues. PCBs are efficacious inducers of drug metabolism and may increase oxidative events and alter many other biochemical and morphologic parameters within cells and tissues. The goal of the present study was to evaluate the effects of a single, very low dose of PCB 126 (3,3′,4,4′,5-pentachlorobiphenyl), a coplanar, dioxin-like PCB congener and aryl hydrocarbon receptor (AhR) agonist, on redox status, metals homeostasis, antioxidant enzymes, and cellular morphology. To examine these parameters, male Sprague–Dawley rats were fed a purified AIN-93 basal diet containing 0.2 ppm selenium for two weeks, then administered a single i.p. injection of corn oil (5 ml/kg body weight) or 1 µmol PCB 126/kg body weight (326 µg/kg body weight) in corn oil. Rats were maintained on the diet for an additional two weeks before being euthanized. This dose of PCB 126 did not alter feed intake or growth, but significantly increased liver weight (42%) and hepatic microsomal cytochrome P-450 (CYP1A) enzyme activities (10–40-fold increase). Hepatic zinc, selenium, and glutathione levels were significantly decreased 15%, 30%, and 20%, respectively, by PCB 126. These changes were accompanied by a 60% decrease in selenium-dependent glutathione peroxidase activity. In contrast, hepatic copper levels were increased 40% by PCB 126. PCB 126-induced pathology was characterized by hepatocellular hypertrophy and mild steatosis in the liver and a mild decrease in cortical T-cells in the thymus. This controlled study in rats fed a purified diet shows that even a single, very low dose of PCB 126 that did not alter feed intake or growth, significantly perturbed redox and metals homeostasis and antioxidant and enzyme levels in rodent liver.

Morphology

Redox status

Selenium

Copper

Polychlorinated biphenyls (PCBs)

Zinc

Glutathione

Details

Title: Subtitle: Acute toxicity of 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) in male Sprague–Dawley rats: Effects on hepatic oxidative stress, glutathione and metals status
Creators: Ian Lai - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, United States
Yingtao Chai - University Hygienic Laboratory, University of Iowa, United States
Don Simmons - University Hygienic Laboratory, University of Iowa, United States
Gregor Luthe - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, United States
Mitchell C Coleman - Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, United States
Douglas Spitz - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, United States
Wanda M Haschek - Department of Pathobiology, University of Illinois, Urbana, IL 61802, United States
Gabriele Ludewig - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, United States
Larry W Robertson - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, United States
Resource Type: Journal article
Publication Details: Environment international, Vol.36(8), pp.918-923
DOI: 10.1016/j.envint.2009.11.002
PMID: 19969354
PMCID: PMC2891598
NLM abbreviation: Environ Int
ISSN: 0160-4120
eISSN: 1873-6750
Publisher: Elsevier Ltd
Grant note: DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences; DOI: 10.13039/100005156, name: Alexander von Humboldt-Stiftung
Language: English
Date published: 2010
Academic Unit: Occupational and Environmental Health; Pathology; Orthopedics and Rehabilitation; Radiation Oncology; Iowa Superfund Research Program
Record Identifier: 9983997459102771

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