Journal article
Acute vaping in a golden Syrian hamster causes inflammatory response transcriptomic changes
American journal of physiology. Lung cellular and molecular physiology, Vol.323(5), pp.L525-L535
Electronic Cigarettes: Not All Good News?
08/30/2022
DOI: 10.1152/ajplung.00162.2022
PMCID: PMC9602905
PMID: 36041220
Abstract
E-cigarette vaping is a major aspect of nicotine consumption, especially for children and young adults. Although it is branded as a safer alternative to cigarette smoking, murine and rat models of subacute and chronic e-cigarette vaping exposure have shown many proinflammatory changes in the respiratory tract. An acute vaping exposure paradigm has not been demonstrated in the golden Syrian hamster, and the hamster is a readily available small animal model that has the unique benefit of becoming infected with and transmitting respiratory viruses, including SARS-CoV-2, without genetic alteration of the animal or virus. Using a 2-day, whole body vaping exposure protocol in male golden Syrian hamsters, we evaluated serum cotinine, bronchoalveolar lavage cells, lung, and nasal histopathology, and gene expression in the nasopharynx and lung through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Depending on the presence of nonnormality or outliers, statistical analysis was performed by ANOVA or Kruskal–Wallis tests. For tests that were statistically significant (
P
< 0.05), post hoc Tukey–Kramer and Dunn’s tests, respectively, were performed to make pairwise comparisons between groups. In nasal tissue, RT-qPCR analysis revealed nicotine-dependent increases in gene expression associated with type 1 inflammation (
CCL-5
and
CXCL-10
), fibrosis [transforming growth factor-β (
TGF-β
)], nicotine-independent increase oxidative stress response (
SOD-2
), and a nicotine-independent decrease in vasculogenesis/angiogenesis (VEGF-A). In the lung, nicotine-dependent increases in the expression of genes involved in the renin-angiotensin pathway [angiotensin-converting enzyme (
ACE
),
ACE2
], coagulation (
tissue factor
,
Serpine-1
), extracellular matrix remodeling (
MMP-2
,
MMP-9
), type 1 inflammation (
IL-1β
,
TNF-α
, and
CXCL-10
), fibrosis (
TGF-β
and
Serpine-1
), oxidative stress response (
SOD-2
), neutrophil extracellular traps release (
ELANE
), and vasculogenesis and angiogenesis (
VEGF-A
) were identified. To our knowledge, this is the first demonstration that the Syrian hamster is a viable model of e-cigarette vaping. In addition, this is the first report that e-cigarette vaping with nicotine can increase
tissue factor
gene expression in the lung. Our results show that even an acute exposure to e-cigarette vaping causes significant upregulation of mRNAs in the respiratory tract from pathways involving the renin-angiotensin system, coagulation, extracellular matrix remodeling, type 1 inflammation, fibrosis, oxidative stress response, neutrophil extracellular trap release (NETosis), vasculogenesis, and angiogenesis.
Details
- Title: Subtitle
- Acute vaping in a golden Syrian hamster causes inflammatory response transcriptomic changes
- Creators
- Daniel M. Hinds - University of IowaHeidi J. Nick - University of Colorado Anschutz Medical CampusTessa M. Vallin - University of Colorado Anschutz Medical CampusLeslie A. Bloomquist - University of Colorado Anschutz Medical CampusSarah Christeson - University of Colorado Anschutz Medical CampusPreston E. Bratcher - University of Colorado Anschutz Medical CampusEmily H. Cooper - University of Colorado Anschutz Medical CampusJohn T. Brinton - University of Colorado Anschutz Medical CampusAngela Bosco-Lauth - Colorado State UniversityCarl W. White - University of Colorado Anschutz Medical Campus
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.323(5), pp.L525-L535
- Publisher
- American Physiological Society
- Series
- Electronic Cigarettes: Not All Good News?
- DOI
- 10.1152/ajplung.00162.2022
- PMID
- 36041220
- PMCID
- PMC9602905
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Grant note
- 3U54ES027698-05S1 / ; U54ES027698-05 / ; CHC R7530S PEDS / ;
- Alternative title
- ACUTE VAPING IN A HAMSTER CAUSES TRANSCRIPTOMIC CHANGES
- Language
- English
- Date published
- 08/30/2022
- Academic Unit
- Pulmonary Medicine; Stead Family Department of Pediatrics
- Record Identifier
- 9984353840302771
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