Journal article
Adaptation of Aerobic, Ethene-Assimilating Mycobacterium Strains to Vinyl Chloride as a Growth Substrate
Environmental science & technology, Vol.42(13), pp.4784-4789
07/01/2008
DOI: 10.1021/es8000536
PMID: 18678006
Abstract
Contamination of drinking water source zones by vinyl chloride (VC), a known human carcinogen and common groundwater contaminant, poses a public health risk. Bioremediation applications involving aerobic, VC-assimilating bacteria could be useful in alleviating environmental VC cancer risk, but their evolution and activity in the environment are poorly understood. In this study, adaptation of ethene-assimilating Mycobacterium strains JS622, JS623, JS624, and JS625 to VC as a growth substrate was investigated to test the hypothesis that VC-assimilating bacteria arise from naturally occurring ethene-assimilating bacteria. VC consumption in the absence of microbial growth was initially observed in cultures grown in both ethene and 1/10-strength trypticase soy agar + 1% (w/v) glucose. After extended incubations (55−476 days), all strains commenced growth-coupled VC consumption patterns. VC-adapted cultures grown on 20 mM acetate subsequently retained their ability to assimilate VC. Three independent purity check methods (streak plates, 16S rRNA gene sequencing, and repetitive extragenic palindromic polymerase chain reaction) verified culture purity prior to and following VC adaptation. Overall, our results suggest that ethene-assimilating mycobacteria have a widespread ability to adapt to VC as a growth substrate.
Details
- Title: Subtitle
- Adaptation of Aerobic, Ethene-Assimilating Mycobacterium Strains to Vinyl Chloride as a Growth Substrate
- Creators
- Yang Oh JinTimothy E Mattes
- Resource Type
- Journal article
- Publication Details
- Environmental science & technology, Vol.42(13), pp.4784-4789
- Publisher
- American Chemical Society
- DOI
- 10.1021/es8000536
- PMID
- 18678006
- ISSN
- 0013-936X
- eISSN
- 1520-5851
- Language
- English
- Date published
- 07/01/2008
- Academic Unit
- Civil and Environmental Engineering
- Record Identifier
- 9983991992402771
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