Journal article
Adaptive Immunity against Listeria monocytogenes in the Absence of Type I Tumor Necrosis Factor Receptor p55
Infection and immunity, Vol.68(8), pp.4470-4476
08/2000
DOI: 10.1128/IAI.68.8.4470-4476.2000
PMCID: PMC98351
PMID: 10899844
Abstract
Tumor necrosis factor (TNF) and the type I TNF receptor (TNFRI), p55, are critical for resistance against primary infections with the intracellular bacterial pathogen
Listeria monocytogenes
. Importantly, however, susceptibility to primary listeriosis in cytokine-deficient mice does not preclude the development or expression of effective adaptive immunity against virulent
L. monocytogenes
. We used TNFRI
−/−
mice to study adaptive antilisterial immunity in the absence of interactions between TNF and TNFRI. Our experiments indicate that TNFRI
−/−
mice survive and clear high-dose challenges with an attenuated strain of
L. monocytogenes
that is incapable of cell-to-cell spread. Furthermore, TNFRI
−/−
mice immunized with attenuated
L. monocytogenes
go on to develop potent adaptive immunity to subsequent high-dose challenges with virulent
L. monocytogenes
. Interestingly, CD8
+
T-cell depletion in vivo inhibits immunity to
L. monocytogenes
in the spleen but not in the liver of TNFRI
−/−
mice. The adaptive immune response in these animals is characterized by activation of listeriolysin O-specific CD8
+
T cells, which are capable of transferring antilisterial immunity to naive wild-type C57BL/6 host mice. These experiments demonstrate the development and expression of potent CD8
+
T-cell-mediated antilisterial immunity in the absence of TNFRI.
Details
- Title: Subtitle
- Adaptive Immunity against Listeria monocytogenes in the Absence of Type I Tumor Necrosis Factor Receptor p55
- Creators
- Douglas W White - Interdisciplinary Graduate Program in ImmunologyVladimir P Badovinac - Interdisciplinary Graduate Program in ImmunologyXin Fan - Interdisciplinary Graduate Program in ImmunologyJohn T Harty - Interdisciplinary Graduate Program in Immunology
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.68(8), pp.4470-4476
- DOI
- 10.1128/IAI.68.8.4470-4476.2000
- PMID
- 10899844
- PMCID
- PMC98351
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 08/2000
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047995802771
Metrics
28 Record Views