Journal article
Adaptive immunity and enhanced CD8+ T cell response to Listeria monocytogenes in the absence of perforin and IFN-gamma
The Journal of immunology (1950), Vol.164(12), pp.6444-6452
06/15/2000
DOI: 10.4049/jimmunol.164.12.6444
PMID: 10843700
Abstract
Single Ag-specific CD8+ T cells from IFN-gamma-deficient (GKO) or perforin-deficient (PKO) mice provide substantial immunity against murine infection with Listeria monocytogenes. To address the potential for redundancy between perforin and IFN-gamma as CD8+ T cell effector mechanisms, we generated perforin/IFN-gamma (PKO/GKO) double-deficient mice. PKO/GKO-derived CD8+ T cells specific for the immunodominant listeriolysin O (LLO91-99) epitope provide immunity to LM infection similar to that provided by Ag-matched wild-type (WT) CD8+ T cells in the liver but reduced in the spleen. Strikingly, polyclonal CD8+ T cells from immunized PKO/GKO mice were approximately 100-fold more potent in reducing bacterial numbers than the same number of polyclonal CD8+ T cells from immunized WT mice. This result is probably quantitative, because the frequency of the CD8+ T cell response against the immunodominant LLO91-99 epitope is >4.5-fold higher in PKO/GKO mice than WT mice at 7 days after identical immunizations. Moreover, PKO/GKO mice can be immunized by a single infection with attenuated Listeria to resist >80,000-fold higher challenges with virulent organisms than naive PKO/GKO mice. These data demonstrate that neither perforin nor IFN-gamma is required for the development or expression of adaptive immunity to LM. In addition, the results suggest the potential for perforin and IFN-gamma to regulate the magnitude of the CD8+ T cell response to infection.
Details
- Title: Subtitle
- Adaptive immunity and enhanced CD8+ T cell response to Listeria monocytogenes in the absence of perforin and IFN-gamma
- Creators
- V P Badovinac - Department of Microbiology and Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City 52242, USAJ T Harty
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.164(12), pp.6444-6452
- DOI
- 10.4049/jimmunol.164.12.6444
- PMID
- 10843700
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- United States
- Grant note
- AI42767 / NIAID NIH HHS AI36864 / NIAID NIH HHS
- Language
- English
- Date published
- 06/15/2000
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047867802771
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