Adenosine increases nasal mucociliary clearance rate in mice through A2A and A2B adenosine receptors

Xiaoyang Hua; Warren C Naselsky; William D Bennett; Catherine Ledent; Brent A Senior; Stephen L Tilley

doi:10.1002/lary.23586

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Adenosine increases nasal mucociliary clearance rate in mice through A2A and A2B adenosine receptors

Journal article

Peer reviewed

Adenosine increases nasal mucociliary clearance rate in mice through A2A and A2B adenosine receptors

Xiaoyang Hua, Warren C Naselsky, William D Bennett, Catherine Ledent, Brent A Senior and Stephen L Tilley

The Laryngoscope, Vol.123(2), pp.306-310

02/2013

DOI: 10.1002/lary.23586

PMID: 22965898

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Abstract

Mucociliary clearance (MCC) is an important mechanism of host defense in the upper and lower respiratory tract. Impaired MCC plays a critical role in the development and perpetuation of chronic rhinosinusitis (CRS). The aim of this investigation was to determine the influence of adenosine on nasal MCC, and to determine the receptors mediating this physiology in vivo. Prospective study using an animal model. Nasal MCC was measured by whole-nose scintigraphic acquisition in vivo. The effects of both endogenous and exogenous adenosine were investigated in wild-type and adenosine receptor knockout (A(2A)(-/-), A(2B)(-/-), A(2A)(-/-)A(2B)(-/-), and A(1)(-/- )A(3)(-/-)) mice. Exogenous adenosine aerosol robustly enhanced nasal MCC. The augmentation of MCC by adenosine was abolished in mice lacking both A(2A) and A(2B) receptors, but remained robust in mice lacking either A(2A) or A(2B) . Likewise, basal nasal MCC was reduced in mice lacking both the A(2A) and A(2B) receptors, but was statistically identical among wild-type mice and mice lacking either A(2A) or A(2B) . These findings indicate that activation of both G(s) -coupled adenosine receptors can accelerate nasal MCC. Targeting these receptors may represent a novel therapeutic approach for enhancing MCC in CRS.

Adenosine - administration & dosage

Adenosine - pharmacology

Administration, Intranasal

Aerosols

Analysis of Variance

Animals

Female

Gamma Cameras

Mice

Mice, Inbred C57BL

Mice, Knockout

Models, Animal

Mucociliary Clearance - drug effects

Prospective Studies

Receptor, Adenosine A2A - drug effects

Receptor, Adenosine A2B - drug effects

Technetium Tc 99m Sulfur Colloid

Details

Title: Subtitle: Adenosine increases nasal mucociliary clearance rate in mice through A2A and A2B adenosine receptors
Creators: Xiaoyang Hua - University of North Carolina at Chapel Hill
Warren C Naselsky - University of North Carolina at Chapel Hill
William D Bennett - University of North Carolina at Chapel Hill
Catherine Ledent - Université Libre de Bruxelles
Brent A Senior - University of North Carolina at Chapel Hill
Stephen L Tilley - University of North Carolina at Chapel Hill
Resource Type: Journal article
Publication Details: The Laryngoscope, Vol.123(2), pp.306-310
DOI: 10.1002/lary.23586
PMID: 22965898
NLM abbreviation: Laryngoscope
ISSN: 0023-852X
eISSN: 1531-4995
Grant note: 1R21AI096139-01A1 / NIAID NIH HHS U19AI07743 / NIAID NIH HHS HL071802 / NHLBI NIH HHS
Language: English
Date published: 02/2013
Academic Unit: Otolaryngology
Record Identifier: 9984311440302771

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