Journal article
Adenovirus-Mediated Gene Therapy for Head and Neck Squamous Cell Carcinomas
Annals of otology, rhinology & laryngology, Vol.105(7), pp.562-567
07/1996
DOI: 10.1177/000348949610500713
PMID: 8678435
Abstract
Advanced head and neck squamous cell carcinomas (HNSCCs) have a poor prognosis despite aggressive multimodal therapy. The goal of our study was to test the feasibility of gene transduction as a novel therapy for head and neck cancer. Three human HNSCC cell lines were transduced in vitro with a replication-deficient recombinant adenovirus containing the lacZ marker gene (Ad2/CMVßgal). Gene transduction efficiency was dependent on multiplicity of infection, duration of exposure to the virus, and viral concentration. Next, the HEp-2 cell line was transduced with an adenoviral vector (Ad.RSVtk) containing the herpes simplex virus thymidine kinase (HSVtk) gene, which, when expressed, sensitizes transduced cells to ganciclovir (GCV). Subsequent administration of GCV resulted in complete rumor cell killing. These results suggest that adenovirus-mediated transfer of KSV tk and administration of GCV has potential as a new therapy for HNSCC.
Details
- Title: Subtitle
- Adenovirus-Mediated Gene Therapy for Head and Neck Squamous Cell Carcinomas
- Creators
- Beverly L Davidson - Iowa City, IowaScott M Graham - Iowa City, IowaEric A Goebel - Iowa City, IowaJoseph Zabner - Iowa City, IowaJeffrey A Kern - Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Annals of otology, rhinology & laryngology, Vol.105(7), pp.562-567
- Publisher
- SAGE Publications; Los Angeles, CA
- DOI
- 10.1177/000348949610500713
- PMID
- 8678435
- ISSN
- 0003-4894
- eISSN
- 1943-572X
- Language
- English
- Date published
- 07/1996
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Neurosurgery; Otolaryngology; Internal Medicine
- Record Identifier
- 9984006343102771
Metrics
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