Journal article
Adenovirus-Mediated Gene Transfer to Normal and Atherosclerotic Arteries: A Novel Approach
Arteriosclerosis, thrombosis, and vascular biology, Vol.15(12), pp.2241-2245
12/1995
DOI: 10.1161/01.ATV.15.12.2241
PMID: 7489249
Abstract
Previous studies of gene transfer to blood vessels in vivo have relied on intraluminal, catheter-based methods for delivery of adenoviral and other vectors. In this study, topical application of a replication-deficient adenoviral vector was used as an alternative method of gene transfer to the vessel wall. We administered recombinant adenovirus (1.0 x 1.5 x 10(10) pfu/mL) containing the nuclear targeted bacterial beta-galactosidase gene topically to arteries in normal and atherosclerotic cynomolgus monkeys. Topical administration was achieved by injection of adenoviral suspension within the periarterial sheath. Segments of femoral and carotid arteries were examined histochemically after staining with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside 1 day after treatment with the adenovirus. After topical administration of virus, beta-galactosidase activity was observed in approximately 20% of cells in the adventitia in both normal and atherosclerotic arteries. There was no detectable beta-galactosidase activity in cells of the intima or media. Thus, topical application provides an alternative method for gene transfer to blood vessels in vivo. This approach, which does not require interruption of blood flow and does not disrupt the endothelium, may be useful for studies of vascular biology and perhaps gene therapy in both normal and atherosclerotic vessels.
Details
- Title: Subtitle
- Adenovirus-Mediated Gene Transfer to Normal and Atherosclerotic Arteries: A Novel Approach
- Creators
- C. David Ríos - From the Departments of Internal Medicine and Pharmacology, Cardiovascular Center and Center on Aging, University of Iowa College of Medicine and Veterans Administration Medical Center, Iowa City, IowaHiroaki Ooboshi - From the Departments of Internal Medicine and Pharmacology, Cardiovascular Center and Center on Aging, University of Iowa College of Medicine and Veterans Administration Medical Center, Iowa City, IowaDonald Piegors - From the Departments of Internal Medicine and Pharmacology, Cardiovascular Center and Center on Aging, University of Iowa College of Medicine and Veterans Administration Medical Center, Iowa City, IowaBeverly L Davidson - From the Departments of Internal Medicine and Pharmacology, Cardiovascular Center and Center on Aging, University of Iowa College of Medicine and Veterans Administration Medical Center, Iowa City, IowaDonald D Heistad - From the Departments of Internal Medicine and Pharmacology, Cardiovascular Center and Center on Aging, University of Iowa College of Medicine and Veterans Administration Medical Center, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Arteriosclerosis, thrombosis, and vascular biology, Vol.15(12), pp.2241-2245
- DOI
- 10.1161/01.ATV.15.12.2241
- PMID
- 7489249
- NLM abbreviation
- Arterioscler Thromb Vasc Biol
- ISSN
- 1079-5642
- eISSN
- 1524-4636
- Language
- English
- Date published
- 12/1995
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040558502771
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