Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimbrial gene products

Douglas B Hornick; Bradley L Allen; Mark A Horn; Steven Clegg

doi:10.1128/IAI.60.4.1577-1588.1992

Back

Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimbrial gene products

Journal article

Open access

Peer reviewed

Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimbrial gene products

Douglas B Hornick, Bradley L Allen, Mark A Horn and Steven Clegg

Infection and immunity, Vol.60(4), pp.1577-1588

04/1992

DOI: 10.1128/IAI.60.4.1577-1588.1992

PMCID: PMC257033

PMID: 1312518

Files and links (1)

url

https://doi.org/10.1128/IAI.60.4.1577-1588.1992View

Published (Version of record) Open Access

Abstract

We examined the role of Klebsiella fimbrial types 1 and 3 in mediating adherence to human buccal and tracheal cells and to lung tissue sections. We found that clinical isolates of Klebsiella pneumoniae producing type 3 fimbriae and Escherichia coli HB101 containing a recombinant plasmid encoding expression of Klebsiella type 3 fimbriae (pFK10) demonstrated increased adherence to tracheal cells, trypsinized buccal cells, and lung tissue sections, in contrast to nonfimbriate and to type 1 fimbriate bacteria. Adherence by type 3 fimbriate bacteria was inhibited by purified type 3 fimbriae and Fab fragments derived from type 3 fimbrial-specific polyclonal immunoglobulin G. Type 3 fimbriae mediated attachment to the basolateral surface of tracheal cells and to the basal epithelial cells and the basement membrane regions of bronchial epithelia. Using an E. coli transformant (pDC17/pFK52), which expresses nonadherent P fimbrial filaments, along with the type 3 fimbrial adhesin (MrkD), we demonstrated that type 3 fimbrial attachment to respiratory cells was attributable to the MrkD adhesin subunit. Subsequent experiments demonstrated that the epithelial target of the type 3 fimbrial adhesin was most likely a peptide molecule rather than a carbohydrate. The results of this study demonstrate that, in vitro, the Klebsiella type 3 fimbrial adhesin mediates adherence to human respiratory tissue.

Lung - microbiology

Fimbriae Proteins

Humans

Acetic Acid

Bacterial Adhesion - physiology

Lactose - pharmacology

Cloning, Molecular

Bacterial Adhesion - drug effects

Polylysine - pharmacology

Immunoglobulin Fab Fragments - physiology

Epithelium - metabolism

Bacterial Proteins - genetics

Chromosome Mapping

Glucose - pharmacology

Adhesins, Bacterial

Recombinant Proteins - pharmacology

Bacterial Proteins - pharmacology

Lysine - pharmacology

Spermine - pharmacology

Escherichia coli - genetics

Klebsiella pneumoniae - pathogenicity

Spermidine - pharmacology

Trachea - microbiology

Acetates - pharmacology

Microbial Collagenase - pharmacology

Protamines - pharmacology

Periodic Acid - pharmacology

In Vitro Techniques

Cheek - microbiology

Details

Title: Subtitle: Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimbrial gene products
Creators: Douglas B Hornick - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242
Bradley L Allen
Mark A Horn
Steven Clegg
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.60(4), pp.1577-1588
DOI: 10.1128/IAI.60.4.1577-1588.1992
PMID: 1312518
PMCID: PMC257033
ISSN: 0019-9567
eISSN: 1098-5522
Grant note: GM07337 / NIGMS NIH HHS HL07638 / NHLBI NIH HHS
Language: English
Date published: 04/1992
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Internal Medicine
Record Identifier: 9984094874402771

Metrics

25 Record Views

76 Times Cited - Web of Science

See more details