Journal article
Adhesion of a Monolayer of Fibroblast Cells to Fibronectin Under Sonic Vibrations in a Bioreactor
Annals of otology, rhinology & laryngology, Vol.121(6), pp.364-374
06/01/2012
DOI: 10.1177/000348941212100602
PMID: 22737958
Abstract
Objectives: We examined cell adhesion to a surface under vibrational forces approximating those of phonation.
Methods: A monolayer of human fibroblast cells was seeded on a fibronectin-coated glass coverslip, which was attached to either the rotating part or the stationary part of a rheometer-bioreactor. The temperature, humidity, carbon dioxide level, nutrients, and cell seeding density were controlled. The cell density was on the order of 1,000 to 5,000 cells per square millimeter. Target stresses above 1 kPa at an oscillatory frequency of 100 Hz were chosen to reflect conditions of vocal fold tissue vibration.
Results: Fibronectin coating provided enough adhesion to support at least 2 kPa of oscillating stress, but only about 0.1 kPa of steady rotational shear. For stresses exceeding those limits, the cells were not able to adhere to the thin film of fibronectin.
Conclusions: Cells will adhere to a planar surface under stresses typical of phonation, which provide a more stringent test than adherence in a 3-dimensional matrix. The density of cell seeding on the coverslip played a role in cell extracellular matrix adhesion, in that the cells adhered to each other more than to the fibronectin coating when the cells were nearly confluent.
Details
- Title: Subtitle
- Adhesion of a Monolayer of Fibroblast Cells to Fibronectin Under Sonic Vibrations in a Bioreactor
- Creators
- Ingo R. Titze - University of IowaSarah A. Klemuk - University of IowaXiaoying Lu - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Annals of otology, rhinology & laryngology, Vol.121(6), pp.364-374
- Publisher
- ANNALS PUBL CO
- DOI
- 10.1177/000348941212100602
- PMID
- 22737958
- ISSN
- 0003-4894
- eISSN
- 1943-572X
- Number of pages
- 11
- Grant note
- R01-DC 4224-05 / National Institute on Deafness and Other Communication Disorders; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD) R01DC004224 / NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD)
- Language
- English
- Date published
- 06/01/2012
- Academic Unit
- School of Music; Communication Sciences and Disorders
- Record Identifier
- 9984719753802771
Metrics
12 Record Views