Journal article
Adipose depot-specific modulation of angiotensinogen gene expression in diet-induced obesity
American journal of physiology: endocrinology and metabolism, Vol.286(6), pp.E891-895
06/2004
DOI: 10.1152/ajpendo.00551.2003
PMID: 14749209
Abstract
Adipose tissue represents an important source of angiotensinogen (AGT). We investigated the effect of obesity induced by a high-fat diet on the expression of mouse (mAGT) and human AGT (hAGT) genes in liver, kidney, and heart and different adipose depots in normal mice (C57BL/6J), and in transgenic mice expressing the hAGT gene under the control of its own promoter. Mice were fed a high-fat diet (45% kcal) or normal chow (10% kcal) for 10 and 20 wk. The expression of mAGT and hAGT mRNA was quantified using an RNAse protection assay. Mice on the high-fat diet exhibited increased weight, fat mass, and plasma leptin. Expression of mAGT or hAGT genes was not affected by high-fat diet in nonadipose tissues, brown adipose tissue, or subcutaneous white fat. In contrast, high-fat diet increased both mAGT and hAGT gene expression in visceral adipose depots (omental, reproductive, and perirenal fat). Thus obesity-induced by a high-fat diet is associated with a tissue-specific increased expression of both mouse and human AGT genes in intra-abdominal adipose tissue. Our findings also suggest that 1.2 kb of regulatory sequences present in the hAGT transgene are sufficient to transcriptionally respond to a high-fat diet in an adipose-specific and depot-specific manner.
Details
- Title: Subtitle
- Adipose depot-specific modulation of angiotensinogen gene expression in diet-induced obesity
- Creators
- Kamal Rahmouni - Hypertension Genetics Specialized Center of Research, Cardiovascular Center, Department of Internal Medical, University of Iowa Carver College of Medicine, Iowa City 52242, USAAllyn L MarkWilliam G HaynesCurt D Sigmund
- Resource Type
- Journal article
- Publication Details
- American journal of physiology: endocrinology and metabolism, Vol.286(6), pp.E891-895
- Publisher
- United States
- DOI
- 10.1152/ajpendo.00551.2003
- PMID
- 14749209
- ISSN
- 0193-1849
- eISSN
- 1522-1555
- Grant note
- HL-48058 / NHLBI NIH HHS HL-61446 / NHLBI NIH HHS HL-14388 / NHLBI NIH HHS HL-44546 / NHLBI NIH HHS HL-55006 / NHLBI NIH HHS
- Language
- English
- Date published
- 06/2004
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040391602771
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