Journal article
Adipose tissue engineering by human adipose-derived stromal cells
Cells, tissues, organs, Vol.183(3), pp.133-140
2006
DOI: 10.1159/000095987
PMID: 17108684
Abstract
Tissue engineering has emerged as a promising alternative approach to current clinical treatments for restoration of soft tissue defects. The purpose of this study was to investigate adipose tissue formation in vitro and in vivo by using human adipose-derived stromal cells (ADSCs) utilizing a gelatin sponge (Gelform) as a scaffold. Adipogenic potentials of human ADSCs were demonstrated by Oil-O-red staining and cellular morphology. After seeding human ADSCs in a density of 3 x 10(6) cells/ml on three-dimensional gelatin sponges, tissue-engineered constructs were exposed to adipogenic differentiation medium for in vitro studies and implanted in the backs of severe combined immunodeficient (SCID) mice for in vivo adipose regeneration. Adipogenesis of ADSC-seeded gelatin sponges was confirmed by Oil-O-red staining after 4 weeks of in vitro incubation. The optical density of the elution from Oil-O-red staining of adipogenic constructs is significantly higher than that of the control group (p < 0.05, n = 4). With short-term in vitro differentiation, adipogenic constructs turned into fat tissue 4 weeks after in vivo implantation, confirmed by biochemical and immunohistochemical examination. No adipogenic-morphological change or fat formation was observed in in vitro or in vivo studies when ADSCs were exposed to a control medium without adipogenic stimulation. These results indicate that engineered adipose tissue can be achieved using human ADSCs and biocompatible and degradable gelatin sponges.
Details
- Title: Subtitle
- Adipose tissue engineering by human adipose-derived stromal cells
- Creators
- Liu Hong - University of Illinois ChicagoIoana A PeptanAylin ColpanJoseph L Daw
- Resource Type
- Journal article
- Publication Details
- Cells, tissues, organs, Vol.183(3), pp.133-140
- DOI
- 10.1159/000095987
- PMID
- 17108684
- NLM abbreviation
- Cells Tissues Organs
- ISSN
- 1422-6405
- eISSN
- 1422-6421
- Language
- English
- Date published
- 2006
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367723702771
Metrics
19 Record Views