Journal article
Adrenergic regulation of ion transport by primary cultures of canine tracheal epithelium: Cellular electrophysiology
The Journal of membrane biology, Vol.91(2), pp.121-128
06/1986
DOI: 10.1007/BF01925789
PMID: 3018255
Abstract
We examined the effect of adrenergic agents on the cellular electrical properties of primary cultures of canine tracheal epithelium. Both isoproterenol and epinephrine stimulated Cl secretion, as evidenced by an increase in transepithelial voltage and a fall in transepithelial resistance. Moreover, both agents appear to increase the conductance of apical and basolateral membranes. However, the pattern of response was different. Isoproterenol initially depolarized apical voltage Ψa and decreased the fractional resistance of the apical membrane fR. These changes are consistent with an initial increase in apical Cl conductance. In contrast, epinephrine acutely hyperpolarized Ψa and increased fR, changes consistent with an initial increase in basolateral K conductance. Following the acute effect of epinephrine, Ψa depolarized and fR decreased to values not significantly different from those observed with isoproterenol. The acute increase in basolateral K conductance produced by epinephrine appeared to result from stimulation of α adrenergic receptors because it was reproduced by addition of the α agonist phenylephrine, and blocked by the α antagonist phentolamine. The ability of prazosin but not yohimbine to block the acute epinephrine-induced increase in K permeability indicates the presence of α1 adrenergic receptors. The acute α adrenergic-induced increase in basolateral K conductance may be mediated by an increase in cell Ca because the response was mimicked by addition of the Ca ionophore A23187. In contrast, the response to isoproterenol was similar to that observed with addition of 8-bromo-cAMP and theophylline. These results indicate that both β and α adrenergic agents mediate the ion transport processes in canine tracheal epithelium. β adrenergic agents have their primary effect on the apical Cl conductance, probably via an increase in cAMP. α adrenergic agents exert their primary effect on the basolateral K conductance, possibly via an increase in cell Ca.
Details
- Title: Subtitle
- Adrenergic regulation of ion transport by primary cultures of canine tracheal epithelium: Cellular electrophysiology
- Creators
- Michael J Welsh
- Resource Type
- Journal article
- Publication Details
- The Journal of membrane biology, Vol.91(2), pp.121-128
- DOI
- 10.1007/BF01925789
- PMID
- 3018255
- ISSN
- 0022-2631
- eISSN
- 1432-1424
- Language
- English
- Date published
- 06/1986
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020890702771
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