Journal article
Adult Stem Cell Driven Genesis of Human-Shaped Articular Condyle
Annals of biomedical engineering, Vol.32(7), pp.911-923
07/01/2004
DOI: 10.1023/B:ABME.0000032454.53116.ee
PMID: 15298429
Abstract
Uniform design of synovial articulations across mammalian species is challenged by their common susceptibility to joint degeneration. The present study was designed to investigate the possibility of creating human-shaped articular condyles by rat bone marrow-derived mesenchymal stem cells (MSCs) encapsulated in a biocompatible poly(ethylene glycol)-based hydrogel. Rat MSCs were harvested, expanded in culture, and treated with either chondrogenic or osteogenic supplements. Rat MSC-derived chondrogenic and osteogenic cells were loaded in hydrogel suspensions in two stratified and yet integrated hydrogel layers that were sequentially photopolymerized in a human condylar mold. Harvested articular condyles from 4-week in vivo implantation demonstrated stratified layers of chondrogenesis and osteogenesis. Parallel in vitro experiments using goat and rat MSCs corroborated in vivo data by demonstrating the expression of chondrogenic and osteogenic markers by biochemical and mRNA analyses. Ex vivo incubated goat MSC-derived chondral constructs contained cartilage-related glycosaminoglycans and collagen. By contrast, goat MSC-derived osteogenic constructs expressed alkaline phosphatase and osteonectin genes, and showed escalating calcium content over time. Rat MSC-derived osteogenic constructs were stiffer than rat MSC-derived chondrogenic constructs upon nanoindentation with atomic force microscopy. These findings may serve as a primitive proof of concept for ultimate tissue-engineered replacement of degenerated articular condyles via a single population of adult mesenchymal stem cells.[PUBLICATION ABSTRACT]
Details
- Title: Subtitle
- Adult Stem Cell Driven Genesis of Human-Shaped Articular Condyle
- Creators
- Adel Alhadlaq - University of Illinois ChicagoJennifer Elisseeff - Johns Hopkins UniversityLiu Hong - University of Illinois ChicagoChristopher Williams - Johns Hopkins UniversityArnold Caplan - Case Western Reserve UniversityBlanka Sharma - Johns Hopkins UniversityRoss Kopher - University of Illinois ChicagoSara Tomkoria - University of Illinois ChicagoDonald Lennon - Case Western Reserve UniversityAurora Lopez - University of Illinois ChicagoJeremy Mao - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Annals of biomedical engineering, Vol.32(7), pp.911-923
- DOI
- 10.1023/B:ABME.0000032454.53116.ee
- PMID
- 15298429
- NLM abbreviation
- Ann Biomed Eng
- ISSN
- 0090-6964
- eISSN
- 1573-9686
- Publisher
- Springer Nature B.V
- Language
- English
- Date published
- 07/01/2004
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367728502771
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