Affect-related behaviors in mice misexpressing the RNA editing enzyme ADAR2

Minati Singh; M Bridget Zimmerman; Terry G Beltz; Alan Kim Johnson

doi:10.1016/j.physbeh.2009.03.029

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Affect-related behaviors in mice misexpressing the RNA editing enzyme ADAR2

Journal article

Open access

Peer reviewed

Affect-related behaviors in mice misexpressing the RNA editing enzyme ADAR2

Minati Singh, M Bridget Zimmerman, Terry G Beltz and Alan Kim Johnson

Physiology & behavior, Vol.97(3-4), pp.446-454

06/22/2009

DOI: 10.1016/j.physbeh.2009.03.029

PMCID: PMC2778280

PMID: 19361536

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http://doi.org/10.1016/j.physbeh.2009.03.029View

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Abstract

Misediting of the serotonin (5HT) 2C receptor (5HT(2C)R) has been implicated in both depression and anxiety. The adenosine deaminases that act on double stranded RNAs (ADARs) are reported to modify the 5HT(2C)R by RNA editing. Transgenic mice misexpressing the RNA editing enzyme ADAR2 show an adult onset obese phenotype due to chronic hyperphagia, but little more than this is known about the behavior of these animals. The present experiments examined whether affect-associated behaviors are also altered in ADAR2 transgenic mice. Age- and weight-matched transgenic mice misexpressing ADAR2 were tested for signs of behavioral despair with the forced swim (FST) and tail suspension (TST) tests, and for anxiety by evaluating spontaneous exploration in a novel environment and by elevated plus maze performance. Plasma corticosterone was also determined by radioimmunoassay. Transgenic mice of both sexes displayed indications of increased behavioral despair on first exposures to the TST and the FST. Behavioral despair persisted in ADAR2 mice in that it was also observed in the FST in tests administered 24 h and 1 week following the initial TST and FST. ADAR2 transgenic mice also displayed behaviors associated with anxiety as indicated by decreased entry into the open arms in an elevated plus maze test. Both sexes of ADAR2 transgenic mice displayed elevated plasma corticosterone. Taken together, the results suggest that ADAR2 transgenic mice represent a novel rodent model of endogenous behavioral despair and anxiety accompanied by elevated hypothalamo-pituitary adrenal axis activity.

Maze Learning - physiology

Adenosine Deaminase - genetics

RNA Editing - genetics

Mice, Inbred C57BL

Genotype

Male

Mice, Transgenic

RNA-Binding Proteins

Hindlimb Suspension - methods

Body Weight - genetics

Affective Symptoms - genetics

Behavior, Animal

Swimming - physiology

Exploratory Behavior - physiology

Motor Activity - genetics

Animals

Analysis of Variance

Female

Mice

Affective Symptoms - blood

Affective Symptoms - physiopathology

Corticosterone - blood

Disease Models, Animal

Details

Title: Subtitle: Affect-related behaviors in mice misexpressing the RNA editing enzyme ADAR2
Creators: Minati Singh - Department of Psychology, University of Iowa, Iowa City, IA 52242, USA
M Bridget Zimmerman
Terry G Beltz
Alan Kim Johnson
Resource Type: Journal article
Publication Details: Physiology & behavior, Vol.97(3-4), pp.446-454
DOI: 10.1016/j.physbeh.2009.03.029
PMID: 19361536
PMCID: PMC2778280
NLM abbreviation: Physiol Behav
ISSN: 0031-9384
eISSN: 1873-507X
Publisher: United States
Grant note: P01 HL014388-350263 / NHLBI NIH HHS HL-079031 / NHLBI NIH HHS HL-70250 / NHLBI NIH HHS R01 DK066086 / NIDDK NIH HHS R01 HL062494-04 / NHLBI NIH HHS R01 HL070250-04 / NHLBI NIH HHS R01 DK066086-05 / NIDDK NIH HHS HL-62494 / NHLBI NIH HHS R01 HL079031 / NHLBI NIH HHS R01 HL070250 / NHLBI NIH HHS HL-14388 / NHLBI NIH HHS R01 HL062494 / NHLBI NIH HHS P01 HL014388 / NHLBI NIH HHS DK-66086 / NIDDK NIH HHS R01 HL079031-02 / NHLBI NIH HHS
Language: English
Date published: 06/22/2009
Academic Unit: Psychological and Brain Sciences; Biostatistics; Neuroscience and Pharmacology; Health, Sport, and Human Physiology ; Internal Medicine
Record Identifier: 9983997475702771

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