Journal article
Affinity Purification of Trypsin Inhibitor with Anti-Aspergillus Flavus Activity from Cultivated and Wild Soybean
Mycopathologia (1975), Vol.167(3), pp.163-171
03/01/2009
DOI: 10.1007/s11046-008-9160-x
PMID: 18830687
Abstract
Trypsin inhibitors (TI) from wild-type soybean (Glycine soya) (WBTI) and domesticated soybean (Glycine max) (SBTI) were purified using prepared chitosan resin-trypsin as filler on the affinity chromatography column. The SBTI/WBTI purification fold by affinity chromatography was 718- and 279-fold, with the activity recovery of 62% and 59%, respectively. It was found that SBTI and WBTI exerted a strong inhibition of Aspergillus. flavus growth, with IC50 of 1.6 and 1.0 mu mol/l. This growth inhibition was possibly the result of the inhibition on alpha-amylase activity of A. flavus by both the SBTI and WBTI. This was further supported by the fact that in the presence of SBTI and WBTI at 9.0 and 6.0 mu g/g (peanut) on peanuts inhibited the germination and growth of A. flavus. Accordingly, characterization of the mode of action of SBTI and WBTI could constitute a first step leading to resistance to A. flavus invasion.
Details
- Title: Subtitle
- Affinity Purification of Trypsin Inhibitor with Anti-Aspergillus Flavus Activity from Cultivated and Wild Soybean
- Creators
- Bin Zhang - Ocean University of ChinaDong-Feng Wang - Ocean University of ChinaYan Fan - Ocean University of ChinaLi Zhang - Ocean University of ChinaYi Luo - Ocean University of China
- Resource Type
- Journal article
- Publication Details
- Mycopathologia (1975), Vol.167(3), pp.163-171
- Publisher
- Springer Nature
- DOI
- 10.1007/s11046-008-9160-x
- PMID
- 18830687
- ISSN
- 0301-486X
- eISSN
- 1573-0832
- Number of pages
- 9
- Grant note
- 200805038 / marine project for public benefits from oceanic bureau of China 2007GG10009005 / Science and Technology Program from Shandong province
- Language
- English
- Date published
- 03/01/2009
- Academic Unit
- Urology
- Record Identifier
- 9984320068802771
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