Journal article
Aggregating platelets increase intracellular calcium in endothelial cells through release of adenine nucleotides
Biochemical and biophysical research communications, Vol.166(2), pp.909-915
01/30/1990
DOI: 10.1016/0006-291X(90)90897-V
PMID: 2302246
Abstract
Aggregating platelets relax isolated coronary arteries through the release of endothelium-derived relaxing factor (EDRF). Since release of EDRF may be calcium dependent, we tested if and how aggregating platelets stimulated a calcium response in cultured endothelial cells. Aggregating platelets caused a transient increase in intracellular calcium in endothelial cells loaded with the fluorescent calcium indicator fura-2. The adenine nucleotides ADP and ATP, but not other platelet-derived mediators, mimicked the platelet-induced calcium response, and inhibition of adenine nucleotides impaired the response to aggregating platelets. Thus, aggregating platelets release adenine nucleotides and stimulate a rise in intracellular calcium in cultured endothelial cells. This calcium response may represent the intracellular transduction mechanism by which aggregating platelets induce endothelial release of EDRF and subsequent relaxation of coronary arteries.
Details
- Title: Subtitle
- Aggregating platelets increase intracellular calcium in endothelial cells through release of adenine nucleotides
- Creators
- Roger C. Ashmore - University of Colorado HealthRichard F. O'Brien - Division of Cardiology Colorado Health Sciences Center, Denver, CO USAThomas J. Stelzner - University of Colorado HealthIra M. Dauber - University of Colorado HealthLawrence D. Horwitz - University of Colorado HealthIvan F. McMurtry - University of Colorado HealthKaryl M. VanBenthuysen - University of Colorado Health
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.166(2), pp.909-915
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0006-291X(90)90897-V
- PMID
- 2302246
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 01/30/1990
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656527402771
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