Journal article
Aging affects isoproterenol-induced water drinking, astrocyte density, and central neuronal activation in female Brown Norway rats
Physiology & behavior, Vol.192, pp.90-97
08/01/2018
DOI: 10.1016/j.physbeh.2018.03.005
PMCID: PMC6019141
PMID: 29518407
Abstract
Age-dependent impairments in the central control of compensatory responses to body fluid challenges have received scant experimental attention, especially in females. In the present study, we found that water drinking in response to β-adrenergic activation with isoproterenol (30 μg/kg, s.c.) was reduced by more than half in aged (25 mo) vs. young (5 mo) ovariectomized female Brown Norway rats. To determine whether this age-related decrease in water intake was accompanied by changes in central nervous system areas associated with fluid balance, we assessed astrocyte density and neuronal activation in the SFO, OVLT, SON, AP and NTS of these rats using immunohistochemical labeling for GFAP and c-fos, respectively. GFAP labeling intensity was increased in the SFO, AP, and NTS of aged females independent of treatment, and was increased in the OVLT of isoproterenol-treated rats independent of age. Fos immunolabeling in response to isoproterenol was reduced in both the SFO and the OVLT of aged females compared to young females, but was increased in the SON of female rats of both ages. Finally, fos labeling in the AP and caudal NTS of aged rats was elevated after vehicle control treatment and did not increase in response to isoproterenol as it did in young females. Thus, age-related declines in water drinking are accompanied by site-specific, age-related changes in astrocyte density and neuronal activation. We suggest that astrocyte density may alter the detection and/or processing of signals related to isoproterenol treatment, and thereby alter neuronal activation in areas associated with fluid balance.
•Isoproterenol-induced water drinking was blunted in aged female Brown Norway rats.•Astrocyte density in most CNS areas involved in body fluid balance was increased in aged females.•Isoproterenol-induced neuronal activation in these areas was differentially affected in aged females.•Thus, aging may alter the detection of or responses to signals related to isoproterenol.
Details
- Title: Subtitle
- Aging affects isoproterenol-induced water drinking, astrocyte density, and central neuronal activation in female Brown Norway rats
- Creators
- Rachel N Hardy - Oklahoma State University – Center of Health Sciences, Tulsa, OK 74107, United StatesZinar D Simsek - Oklahoma State University – Center of Health Sciences, Tulsa, OK 74107, United StatesBrandon Curry - Oklahoma State University – Center of Health Sciences, Tulsa, OK 74107, United StatesSheri L Core - Oklahoma State University – Center of Health Sciences, Tulsa, OK 74107, United StatesTerry Beltz - University of Iowa, Iowa City, IA, United StatesBaojian Xue - University of Iowa, Iowa City, IA, United StatesAlan K Johnson - University of Iowa, Iowa City, IA, United StatesRobert L Thunhorst - University of Iowa, Iowa City, IA, United StatesKathleen S Curtis - Oklahoma State University – Center of Health Sciences, Tulsa, OK 74107, United States
- Resource Type
- Journal article
- Publication Details
- Physiology & behavior, Vol.192, pp.90-97
- DOI
- 10.1016/j.physbeh.2018.03.005
- PMID
- 29518407
- PMCID
- PMC6019141
- NLM abbreviation
- Physiol Behav
- ISSN
- 0031-9384
- eISSN
- 1873-507X
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100008569, name: Oklahoma Center for the Advancement of Science and Technology, award: 12-196; name: OK INBRE Program, award: 8P20GM103447, AG25465, HL098207, MH80241, HL14388
- Language
- English
- Date published
- 08/01/2018
- Academic Unit
- Psychological and Brain Sciences; Neuroscience and Pharmacology; Neurology (Pediatrics); Health, Sport, and Human Physiology
- Record Identifier
- 9984002598102771
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