Journal article
Aging and infection reduce expression of specific brain-derived neurotrophic factor mRNAs in hippocampus
Neurobiology of aging, Vol.33(4), pp.832.e1-832.e14
04/01/2012
DOI: 10.1016/j.neurobiolaging.2011.07.015
PMCID: 3237944
PMID: 21907460
Abstract
Aging increases the likelihood of cognitive decline after negative life events such as infection or injury. We have modeled this increased vulnerability in aged (24-month-old), but otherwise unimpaired F344xBN rats. In these animals, but not in younger (3-month-old) counterparts, a single intraperitoneal injection of E. coli leads to specific deficits in long-term memory and long-lasting synaptic plasticity in hippocampal area CA1-processes strongly dependent on brain-derived neurotrophic factor (BDNF). Here we have investigated the effects of age and infection on basal and fear-conditioning-stimulated expression of Bdnf in hippocampus. We performed in situ hybridization with 6 probes recognizing: total (pan-)BDNF mRNA, the 4 predominant 5' exon-specific transcripts (I, II, IV, and VI), and BDNF mRNAs with a long 3' untranslated region (3' UTR). In CA1, aging reduced basal levels and fear-conditioning-induced expression of total BDNF mRNA, exon IV-specific transcripts, and transcripts with long 3' UTRs; effects of infection were similar and sometimes compounded the effects of aging. In CA3, aging reduced all of the transcripts to some degree; infection had no effect. Effects in dentate were minimal. Northern blot analysis confirmed an aging-associated loss of total BDNF mRNA in areas CA1 and CA3, and revealed a parallel, preferential loss of BDNF mRNA transcripts with long 3' UTRs.
Details
- Title: Subtitle
- Aging and infection reduce expression of specific brain-derived neurotrophic factor mRNAs in hippocampus
- Creators
- Timothy R. Chapman - Department of Psychology and Neuroscience and The Center for Neuroscience, University of Colorado, Boulder, CO 80309, USARuth M. BarrientosJared T. AhrendsenJennifer M HooverSteven F MaierSusan Patterson
- Resource Type
- Journal article
- Publication Details
- Neurobiology of aging, Vol.33(4), pp.832.e1-832.e14
- DOI
- 10.1016/j.neurobiolaging.2011.07.015
- PMID
- 21907460
- PMCID
- 3237944
- ISSN
- 0197-4580
- eISSN
- 1558-1497
- Grant note
- DOI: 10.13039/100010174, name: University of Colorado; DOI: 10.13039/100000049, name: National Institute on Aging, award: 1R21AG031467, 1R01AG02827
- Language
- English
- Date published
- 04/01/2012
- Academic Unit
- Iowa Neuroscience Institute
- Record Identifier
- 9984454746502771
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