Journal article
Aging reduces responsiveness to BSO- and heat stress-induced perturbations of glutathione and antioxidant enzymes
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.289(4), pp.R1035-1041
10/2005
DOI: 10.1152/ajpregu.00254.2005
PMID: 15947071
Abstract
Aging alters cellular responses to both heat and oxidative stress. Thiol-mediated metabolism of reactive oxygen species (ROS) is believed to be important in aging. To begin to determine the role of thiols in aging and heat stress, we depleted liver glutathione (GSH) by administering l-buthionine sulfoximine (BSO) in young (6 mo) and old (24 mo) Fisher 344 rats before heat stress. Animals were given BSO (4 mmol/kg ip) or saline (1 ml ip) 2 h before heat stress and subsequently heated to a core temperature of 41 degrees C over a 90-min period. Liver tissue was collected before and 0, 30, and 60 min after heat stress. BSO inhibited glutamate cysteine ligase (GCL, the rate-limiting enzyme in GSH synthesis) catalytic activity and resulted in a decline in liver GSH and GSSG that was more pronounced in young compared with old animals. Catalase activity did not change between groups until 60 min after heat stress in young BSO-treated rats. Young animals experienced a substantial and persistent reduction in Cu,Zn-SOD activity with BSO treatment. Mn-SOD activity increased with BSO but declined after heat stress. The differences in thiol depletion observed between young and old animals with BSO treatment may be indicative of age-related differences in GSH compartmentalization that could have an impact on maintenance of redox homeostasis and antioxidant balance immediately after a physiologically relevant stress. The significant changes in antioxidant enzyme activity after GSH depletion suggest that thiol status can influence the regulation of other antioxidant enzymes.
Details
- Title: Subtitle
- Aging reduces responsiveness to BSO- and heat stress-induced perturbations of glutathione and antioxidant enzymes
- Creators
- Joanna P Morrison - Integrative Physiology Laboratory, 532 FH, Department of Exercise Science, The University of Iowa, Iowa City, IA 52242, USAMitchell C ColemanElizabeth S AunanSusan A WalshDouglas R SpitzKevin C Kregel
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.289(4), pp.R1035-1041
- Publisher
- United States
- DOI
- 10.1152/ajpregu.00254.2005
- PMID
- 15947071
- ISSN
- 0363-6119
- eISSN
- 1522-1490
- Grant note
- R01-AG-12350 / NIA NIH HHS R01-HL-51469 / NHLBI NIH HHS P01-CA-66081 / NCI NIH HHS R01-CA-100045 / NCI NIH HHS
- Language
- English
- Date published
- 10/2005
- Academic Unit
- Radiology; Pathology; Orthopedics and Rehabilitation; Radiation Oncology; Provost Office Administration; Health and Human Physiology
- Record Identifier
- 9984024507602771
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