Journal article
Airway Caliber is Linked to Wall Thickness in Mice: Implications for Morphometry
The FASEB journal, Vol.33(S1), pp.lb358-lb358
04/2019
DOI: 10.1096/fasebj.2019.33.1_supplement.lb358
Abstract
Morphometry of lung tissues often involves evaluation for changes in airway wall components such as collagen and/or smooth muscle. Alterations of these tissue parameters may be markers of diseases like asthma, chronic obstructive pulmonary disease or cystic fibrosis. The mouse lung is composed of airways that exhibit monopodial branching during development and these intrapulmonary airways are exclusively bronchioles (i.e. no intra‐pulmonary bronchi). As such, many airways can look alike for analysis by inexperienced observers. We tested if the airway caliber in wild type B6 mice could influence the detection and scoring of airway collagen or smooth muscle composition. We examined 15 lungs from archival blocks that came from studies with appropriate institutional approvals. Airway lumen diameter as well as wall thickness (sum of epithelium, smooth muscle and collagen) measurements of the 2 largest and 2 smallest airways were taken. These were then cumulatively analyzed for wall thickness (y axis) and airway lumen diameter (x axis). There was a significant positive slope (P=0.0017). We then grouped the data into large (lumen >130 microns) and small (lumen <130 microns) airways and compared the wall thickness, which was significantly thicker in the larger airways (P<0.0001). These data indicate that morphometry of murine airways requires analysis of similar sized airways for accurate assessment of changes and we speculate this finding will be relevant across other species as well.
Support or Funding Information
NA
This is from the Experimental Biology 2019 Meeting. There is no full text article associated with this published in The FASEB Journal.
Details
- Title: Subtitle
- Airway Caliber is Linked to Wall Thickness in Mice: Implications for Morphometry
- Creators
- David K Meyerholz - University of IowaAmanda P Beck - Albert Einstein College of Medicine
- Resource Type
- Journal article
- Publication Details
- The FASEB journal, Vol.33(S1), pp.lb358-lb358
- Publisher
- The Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.2019.33.1_supplement.lb358
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 04/2019
- Academic Unit
- Pathology
- Record Identifier
- 9984201114602771
Metrics
7 Record Views