Journal article
Airway Surface Liquid Has Innate Antiviral Activity That Is Reduced in Cystic Fibrosis
American journal of respiratory cell and molecular biology, Vol.62(1), pp.104-111
01/2020
DOI: 10.1165/rcmb.2018-0304OC
PMCID: PMC6938132
PMID: 31242392
Abstract
Although chronic bacterial infections and inflammation are associated with progressive lung disease in patients with cystic fibrosis (CF), much less is known regarding the contributions of respiratory viral infections to this process. Clinical studies suggest that antiviral host defenses may be compromised in individuals with CF, and CF airway epithelia exhibit impaired antiviral responses
. Here, we used the CF pig model to test the hypothesis that the antiviral activity of respiratory secretions is reduced in CF. We developed an
assay to measure the innate antiviral activity present in airway surface liquid (ASL) from CF and non-CF pigs. We found that tracheal and nasal ASL from newborn non-CF pigs exhibited dose-dependent inhibitory activity against several enveloped and encapsidated viruses, including Sendai virus, respiratory syncytial virus, influenza A, and adenovirus. Importantly, we found that the anti-Sendai virus activity of nasal ASL from newborn CF pigs was significantly diminished relative to non-CF littermate controls. This diminution of extracellular antiviral defenses appears to be driven, at least in part, by the differences in pH between CF and non-CF ASL. These data highlight the novel antiviral properties of native airway secretions and suggest the possibility that defects in extracellular antiviral defenses contribute to CF pathogenesis.
Details
- Title: Subtitle
- Airway Surface Liquid Has Innate Antiviral Activity That Is Reduced in Cystic Fibrosis
- Creators
- Abigail R Berkebile - Department of Microbiology and Immunology.Jennifer A Bartlett - Department of Pediatrics.Mahmoud Abou Alaiwa - Department of Internal Medicine, andSteven M Varga - Roy J. and Lucille A. Carver College of MedicineUltan F Power - Queen's University BelfastPaul B McCray Jr - Department of Pediatrics
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.62(1), pp.104-111
- DOI
- 10.1165/rcmb.2018-0304OC
- PMID
- 31242392
- PMCID
- PMC6938132
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Grant note
- P30 ES005605 / NIEHS NIH HHS P30 DK054759 / NIDDK NIH HHS P01 HL091842 / NHLBI NIH HHS
- Language
- English
- Date published
- 01/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pulmonary, Critical Care, and Occupational Medicine; Graduate College Admin and Gen; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Pathology; Internal Medicine
- Record Identifier
- 9984297323702771
Metrics
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