Journal article
Airway surface fluid volume and Cl content in cystic fibrosis and normal bronchial xenografts
American Journal of Physiology: Cell Physiology, Vol.276(2), pp.C469-C476
02/01/1999
DOI: 10.1152/ajpcell.1999.276.2.C469
PMID: 9950775
Abstract
We describe the use of an in vivo human bronchial xenograft model of cystic fibrosis (CF) and non-CF airways to investigate pathophysiological alterations in airway surface fluid (ASF) volume (Vs) and Cl content. Vs was calculated based on the dilution of an impermeable marker, [3H]inulin, during harvesting of ASF from xenografts with an isosmotic Cl-free solution. These calculations demonstrated that Vs in CF xenographs (28 ± 3.0 μl/cm2; n = 17) was significantly less than that of non-CF xenografts (35 ± 2.4 μl/cm2; n = 30). The Cl concentration of ASF ([Cl]s) was determined using a solid-state AgCl electrode and adjusted for dilution during harvesting using the impermeable [3H]inulin marker. Cumulative results demonstrate small but significant elevations ( P < 0.045) in [Cl]s in CF (125 ± 4 mM; n = 27) compared with non-CF (114 ± 4 mM; n = 48) xenografts. To investigate potential mechanisms by which CF airways may facilitate a higher level of fluid absorption yet retain slightly elevated levels of Cl, we sought to evaluate the capacity of CF and non-CF airways to absorb both 22Na and36Cl. Two consistent findings were evident from these studies. First, in both CF and non-CF xenografts,22Na and36Cl were always absorbed in an equal molar ratio. Second, CF xenografts hyperabsorbed (∼1.5-fold higher) both 22Na and36Cl compared with non-CF xenografts. These results substantiate previously documented findings of elevated Na absorption in CF airways and also suggest that the slightly elevated [Cl]s found in this study of CF xenograft epithelia does not occur through a mechanism of decreased apical permeability to Cl.
Details
- Title: Subtitle
- Airway surface fluid volume and Cl content in cystic fibrosis and normal bronchial xenografts
- Creators
- Yulong Zhang - Departments of Anatomy and Cell Biology and of Internal Medicine, University of Iowa Medical School, Iowa City, Iowa 52242John F Engelhardt - Departments of Anatomy and Cell Biology and of Internal Medicine, University of Iowa Medical School, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- American Journal of Physiology: Cell Physiology, Vol.276(2), pp.C469-C476
- DOI
- 10.1152/ajpcell.1999.276.2.C469
- PMID
- 9950775
- ISSN
- 0363-6143
- eISSN
- 1522-1563
- Language
- English
- Date published
- 02/01/1999
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025570002771
Metrics
27 Record Views