Journal article
Alcaligenes faecalis corrects aberrant matrix metalloproteinase expression to promote reepithelialization of diabetic wounds
Science advances, Vol.10(26), eadj2020
06/28/2024
DOI: 10.1126/sciadv.adj2020
PMCID: PMC11204295
PMID: 38924411
Abstract
Chronic wounds are a common and costly complication of diabetes, where multifactorial defects contribute to dysregulated skin repair, inflammation, tissue damage, and infection. We previously showed that aspects of the diabetic foot ulcer microbiota were correlated with poor healing outcomes, but many microbial species recovered remain uninvestigated with respect to wound healing. Here, we focused on
Alcaligenes faecalis
, a Gram-negative bacterium that is frequently recovered from chronic wounds but rarely causes infection. Treatment of diabetic wounds with
A. faecalis
accelerated healing during early stages. We investigated the underlying mechanisms and found that
A. faecalis
treatment promotes reepithelialization of diabetic keratinocytes, a process that is necessary for healing but deficient in chronic wounds. Overexpression of matrix metalloproteinases in diabetes contributes to failed epithelialization, and we found that
A. faecalis
treatment balances this overexpression to allow proper healing. This work uncovers a mechanism of bacterial-driven wound repair and provides a foundation for the development of microbiota-based wound interventions.
A bacteria commonly found in chronic wounds accelerates wound closure by correcting a pathway that is dysregulated in diabetes.
Details
- Title: Subtitle
- Alcaligenes faecalis corrects aberrant matrix metalloproteinase expression to promote reepithelialization of diabetic wounds
- Creators
- Ellen K. White - University of PennsylvaniaAayushi Uberoi - University of PennsylvaniaJamie Ting-Chun Pan - University of PennsylvaniaJordan T. Ort - University of PennsylvaniaAmy E. Campbell - University of PennsylvaniaSofia M. Murga-Garrido - University of PennsylvaniaJordan C. Harris - University of PennsylvaniaPreeti Bhanap - University of PennsylvaniaMonica Wei - University of PennsylvaniaNelida Y. Robles - University of PennsylvaniaSue E. Gardner - University of IowaElizabeth A. Grice - University of Pennsylvania
- Resource Type
- Journal article
- Publication Details
- Science advances, Vol.10(26), eadj2020
- Publisher
- American Association for the Advancement of Science
- DOI
- 10.1126/sciadv.adj2020
- PMID
- 38924411
- PMCID
- PMC11204295
- ISSN
- 2375-2548
- eISSN
- 2375-2548
- Grant note
- R01NR015639 / ; F31AR079852 / ; 5T32AI141393 / ; ; T32AR007465 / ; R01NR009448 / ; F31AR079845 / ; K99 AR081404 / ; R25 GM071745-19 / ; Awesome Games Done Quick Fellowship / ; P30AR069589 / ;
- Alternative title
- Microbial mechanism to promote reepithelialization of diabetic wounds
- Language
- English
- Date published
- 06/28/2024
- Academic Unit
- Nursing
- Record Identifier
- 9984649044502771
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