Journal article
Alcian Blue and Pyronine Y Histochemical Stains Permit Assessment of Multiple Parameters in Pulmonary Disease Models
Veterinary pathology, Vol.46(2), pp.325-328
03/2009
DOI: 10.1354/vp.46-2-325
PMCID: PMC2802330
PMID: 19261646
Abstract
Utilization of a combined Alcian Blue and Pyronine Y histochemical method for the assessment of multiple parameters in the respiratory tract of various species is described. Acidic mucins were deep blue (sialylated mucins), red (sulfated mucins), or variably purple (mixture of sialylated/sulfated mucins), and differential mucus production was readily detected in a murine respiratory syncytial virus vaccine model of pulmonary inflammation. Elastic fibers stained red in the walls of pulmonary arteries, connecting airways, alveolar septa, and subpleural interstitium. Mast cells had red to red-purple granular cytoplasmic staining. Nuclei were ubiquitously counterstained pale blue. Representative staining was detected in tissues from multiple species, including inbred mice, rats, ferrets, cats, dogs, sheep, and pigs. The fluorescent property of the stained tissues offers additional modalities with which to analyze tissue sections. This histochemical technique detects multiple critical parameters in routine paraffin sections of lung tissue, reduces the need for repeated serial sectioning and staining, and is cost-effective and simple to perform.
Details
- Title: Subtitle
- Alcian Blue and Pyronine Y Histochemical Stains Permit Assessment of Multiple Parameters in Pulmonary Disease Models
- Creators
- D. K Meyerholz - Departments of Pathology University of Iowa, Iowa City, IAJ Rodgers - Departments of Pathology University of Iowa, Iowa City, IAE. M Castilow - Roy J. and Lucille A. Carver College of Medicine; and Interdisciplinary Graduate Program in Immunology (EMC, SMV), University of Iowa, Iowa City, IAS. M Varga - Microbiology University of Iowa, Iowa City, IA, Roy J. and Lucille A. Carver College of Medicine; and Interdisciplinary Graduate Program in Immunology (EMC, SMV), University of Iowa, Iowa City, IA
- Resource Type
- Journal article
- Publication Details
- Veterinary pathology, Vol.46(2), pp.325-328
- DOI
- 10.1354/vp.46-2-325
- PMID
- 19261646
- PMCID
- PMC2802330
- NLM abbreviation
- Vet Pathol
- ISSN
- 0300-9858
- eISSN
- 1544-2217
- Language
- English
- Date published
- 03/2009
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083886102771
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