Journal article
Alcoholic and isocaloric diet, but not ovariectomy, influence the apoptosis of bone cells within the alveolar bone crest of rats
Archives of Oral Biology, Vol.59(4), pp.424-433
2014
DOI: 10.1016/j.archoralbio.2014.01.008
PMID: 24549089
Abstract
Objective: Studies suggest that chronic alcoholism as well as oestrogen deficiencies may affect bones in general, including alveolar bone and, by doing so, increase individuals’ susceptibility to develop progressive periodontal disease. This paper aims to verify the influence of chronic alcoholism and/or oestrogen deficiencies in the apoptosis of bone cells of the alveolar bone crest region in rats. Design: Initially, 54 rats were divided into ovariectomized (Ovx) and Sham operated (Sham) groups. Thirty days after surgery, these two groups were equally sub-divided, and received, for 56 days, the following dietary intervention: alcoholic diet (with 20% alcohol solution,), isocaloric diet and ad libitum diet (free diet). Analysis was undertaken by immunohistochemistry, using an antibody to detect apoptosis (anti PARP p-85). Results: When comparing the six experimental groups, no significant differences were observed in the apoptosis of bone cells. Also, there was no significant difference in the quantity of cells undergoing apoptosis when the animals from Ovx groups were compared with those from Sham groups. However, when comparing only different dietary groups, differences were observed between the groups ad libitum and isocaloric, to osteoblasts ( p = 0.045); and ad libitum and alcohol, to osteocytes ( p = 0.007). Conclusion: It is concluded that ovariectomy was not able to influence the rate of apoptosis of bone cells of the alveolar bone crest region in rats and that a possible influence of diet on apoptosis of osteoblasts and osteocytes cannot be ruled out.
Details
- Title: Subtitle
- Alcoholic and isocaloric diet, but not ovariectomy, influence the apoptosis of bone cells within the alveolar bone crest of rats
- Creators
- Adriana Mathias Pereira da Silva Marchini - University of Iowa, Preventive and Community DentistryLucélia Lemes GonçalvesMiriane Carneiro Machado SalgadoRenata Falchete do PradoLeonardo Marchini - University of Iowa, Preventive and Community DentistryYasmin Rodarte CarvalhoRosilene Fernandes da Rocha
- Resource Type
- Journal article
- Publication Details
- Archives of Oral Biology, Vol.59(4), pp.424-433
- DOI
- 10.1016/j.archoralbio.2014.01.008
- PMID
- 24549089
- ISSN
- 0003-9969
- eISSN
- 1879-1506
- Language
- English
- Date published
- 2014
- Academic Unit
- Preventive and Community Dentistry
- Record Identifier
- 9983765985302771
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