Aldehyde dehydrogenase 3A1 protects airway epithelial cells from cigarette smoke-induced DNA damage and cytotoxicity

Jun-Ho Jang; Shannon Bruse; Yushi Liu; Veronica Duffy; Chunyu Zhang; Nathaniel Oyamada; Scott Randell; Akiko Matsumoto; David C. Thompson; Yong Lin; Vasilis Vasiliou; Yohannes Tesfaigzi; Toru Nyunoya

doi:10.1016/j.freeradbiomed.2013.11.028

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Aldehyde dehydrogenase 3A1 protects airway epithelial cells from cigarette smoke-induced DNA damage and cytotoxicity

Journal article

Peer reviewed

Aldehyde dehydrogenase 3A1 protects airway epithelial cells from cigarette smoke-induced DNA damage and cytotoxicity

Jun-Ho Jang, Shannon Bruse, Yushi Liu, Veronica Duffy, Chunyu Zhang, Nathaniel Oyamada, Scott Randell, Akiko Matsumoto, David C. Thompson, Yong Lin, …

Free radical biology & medicine, Vol.68, pp.80-86

03/01/2014

DOI: 10.1016/j.freeradbiomed.2013.11.028

PMID: 24316006

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Abstract

Aldehyde dehydrogenase 3A1 (ALDH3A1), an ALDH superfamily member, catalyzes the oxidation of reactive aldehydes, highly toxic components of cigarette smoke (CS). Even so, the role of ALDH3A1 in CS-induced cytotoxicity and DNA damage has not been examined. Among all of the ALDH superfamily members, ALDH3A1 mRNA levels showed the greatest induction in response to CS extract (CSE) exposure of primary human bronchial epithelial cells (HBECs). ALDH3A1 protein accumulation was accompanied by increased ALDH enzymatic activity in CSE-exposed immortalized HBECs. The effects of overexpression or suppression of ALDH3A1 on CSE-induced cytotoxicity and DNA damage (γH2AX) were evaluated in cultured immortalized HBECs. Enforced expression of ALDH3A1 attenuated cytotoxicity and downregulated γH2AX. SiRNA-mediated suppression of ALDH3A1 blocked ALDH enzymatic activity and augmented cytotoxicity in CSE-exposed cells. Our results suggest that the availability of ALDH3A1 is important for cell survival against CSE in HBECs.

Airway epithelial cells

ALDH3A1

Chronic obstructive pulmonary disease

Cigarette smoke

Cytotoxicity

DNA damage

FANCD2

Free radicals

Reactive aldehydes

Details

Title: Subtitle: Aldehyde dehydrogenase 3A1 protects airway epithelial cells from cigarette smoke-induced DNA damage and cytotoxicity
Creators: Jun-Ho Jang - Lovelace Respiratory Research Institute
Shannon Bruse - Lovelace Respiratory Research Institute
Yushi Liu - Lovelace Respiratory Research Institute
Veronica Duffy - Lovelace Respiratory Research Institute
Chunyu Zhang - Lovelace Respiratory Research Institute
Nathaniel Oyamada - Lovelace Respiratory Research Institute
Scott Randell - University of North Carolina at Chapel Hill
Akiko Matsumoto - University of Colorado Denver
David C. Thompson - University of Colorado Denver
Yong Lin - Lovelace Respiratory Research Institute
Vasilis Vasiliou - University of Colorado Denver
Yohannes Tesfaigzi - Lovelace Respiratory Research Institute
Toru Nyunoya - Lovelace Respiratory Research Institute
Resource Type: Journal article
Publication Details: Free radical biology & medicine, Vol.68, pp.80-86
DOI: 10.1016/j.freeradbiomed.2013.11.028
PMID: 24316006
ISSN: 0891-5849
eISSN: 1873-4596
Publisher: Elsevier Inc
Number of pages: 7
Language: English
Date published: 03/01/2014
Academic Unit: Internal Medicine
Record Identifier: 9985214172002771

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