Journal article
Alkaline, Endo III and FPG modified comet assay as biomarkers for the detection of oxidative DNA damage in rats with experimentally induced diabetes
Mutation research. Genetic toxicology and environmental mutagenesis, Vol.726(2), pp.242-250
12/24/2011
DOI: 10.1016/j.mrgentox.2011.10.004
PMID: 22015262
Abstract
Increased production of reactive oxygen species under diabetic condition underlines the higher oxidatively damaged DNA in different tissues. However, it is practically difficult to assess the oxidatively damaged DNA in different internal organs. Therefore, the present study was aimed to evaluate the extent of oxidative stress-induced DNA damage in different organs with the progression of diabetes. Diabetic and control Sprague Dawley rats were sacrificed in time-dependent manner and the lung, liver, heart, aorta, kidney, pancreas and peripheral blood lymphocytes (PBL) were analyzed for both alkaline and modified comet assay with endonuclease-III (Endo III) and formamidopyrimidine-DNA glycosylase (FPG) (hereafter called modified comet assay) for the detection of oxidative DNA damage. The statistically significant increase in olive tail moment (OTM) was found in all the tested tissues. The extent of DNA damage was increased with the progression of diabetes as revealed by the parameter of OTM in alkaline and modified comet assay. Further, the positive correlations were observed between OTM of the lung, liver, heart, aorta, kidney and pancreas with PBL of diabetic rat in the alkaline and modified comet assay. Moreover, significant increase in the 8-oxodG positive nuclei in the lung, liver, heart, aorta, kidney and pancreas was observed in 4th and 8th week diabetic rat as compared to control. Results of the present study clearly indicated the suitability of alkaline and modified comet assay for the detection of multi-organ oxidative DNA damage in streptozotocin (STZ)-induced diabetic rat and showed that damaged DNA of PBL can be used as a suitable biomarker to assess the internal organs response to DNA damage in diabetes. (C) 2011 Elsevier B.V. All rights reserved.
Details
- Title: Subtitle
- Alkaline, Endo III and FPG modified comet assay as biomarkers for the detection of oxidative DNA damage in rats with experimentally induced diabetes
- Creators
- S. Kushwaha - Natl Inst Pharmaceut Educ & Res, Facil Risk Assessment & Intervent Studies, Dept Pharmacol & Toxicol, Sect 67, Sas Nagar 160062, Punjab, IndiaA. Vikram - National Institute of Pharmaceutical Education and ResearchP. P. Trivedi - Natl Inst Pharmaceut Educ & Res, Facil Risk Assessment & Intervent Studies, Dept Pharmacol & Toxicol, Sect 67, Sas Nagar 160062, Punjab, IndiaG. B. Jena - National Institute of Pharmaceutical Education and Research
- Resource Type
- Journal article
- Publication Details
- Mutation research. Genetic toxicology and environmental mutagenesis, Vol.726(2), pp.242-250
- Publisher
- Elsevier
- DOI
- 10.1016/j.mrgentox.2011.10.004
- PMID
- 22015262
- ISSN
- 1383-5718
- eISSN
- 1879-3592
- Number of pages
- 9
- Grant note
- National Institute of Pharmaceutical Education and Research (NIPER), S.A.S. Nagar, Mohali, Punjab, India SR/SO/HS-83/2008 / Department of Science and Technology, Govt. of India; Department of Science & Technology (India)
- Language
- English
- Date published
- 12/24/2011
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984359763802771
Metrics
23 Record Views