Alpha 1B-adrenergic receptors in rat renal microvessels

Leonardo M Canessa; Marlene M Piccio; Prayong Vachvanichsanong; Anita Sidhu; Craig C Porter; Jean E Robillard; Robin A Felder; Pedro A Jose

doi:10.1038/ki.1995.430

Back

Alpha 1B-adrenergic receptors in rat renal microvessels

Journal article

Open access

Peer reviewed

Alpha 1B-adrenergic receptors in rat renal microvessels

Leonardo M Canessa, Marlene M Piccio, Prayong Vachvanichsanong, Anita Sidhu, Craig C Porter, Jean E Robillard, Robin A Felder and Pedro A Jose

Kidney international, Vol.48(5), pp.1412-1419

11/1995

DOI: 10.1038/ki.1995.430

PMID: 8544397

Files and links (1)

url

https://doi.org/10.1038/ki.1995.430View

Published (Version of record) Open Access

Abstract

Although several alpha-adrenergic receptor genes are expressed in the rat kidney, their expression in the renal vasculature has not been studied. Since pharmacological studies have suggested that an alpha 1B-adrenergic receptor may mediate renal vasoconstriction, we studied the expression of alpha 1B-adrenergic receptors in renal microvessels, from 10- to 14-week-old male spontaneously hypertensive rats (SHR) and their normotensive control, the Wistar-Kyoto rat (WKY). In these microvessels, isolated by perfusion with iron, alpha 1B-adrenergic receptor mRNA levels (by ribonuclease protection assay) were similar in SHR and WKY rats. Photo-affinity labeling with [125I]-arylazidoprazosin demonstrated the presence of alpha 1B-adrenergic receptor protein. Maximum receptor density (determined by 3H-prazosin binding: Bmax 59.8 +/- 4.1 and 58.7 +/- 4.3; Kd 0.48 +/- 0.05 nM and 0.31 +/- 0.06 nM in SHR and WKY, respectively) and chloroethylclonidine (CEC)-sensitive binding sites (determined by [125I]-(2-beta(4-hydroxyphenyl)-ethylaminomethyl)-tetralone binding) (125I-HEAT) were similar in SHR and WKY rats. There are two novel findings in these studies: (1) the alpha 1B-adrenergic receptor gene is expressed in renal microvessels of WKY and SHR; (2) alpha 1B-adrenergic receptor gene expression in renal microvessels is not altered in adult SHR. The failure to down-regulate expression of the alpha 1B-adrenergic receptor at the mRNA and protein level in the SHR could result in persistence of alpha 1B-adrenergic receptor effects and contribute to the increased vascular resistance in hypertension.

Affinity Labels

Rats, Inbred SHR

Receptors, Adrenergic, alpha - metabolism

Alkylating Agents - pharmacology

Blood Vessels - metabolism

Rats

Rats, Inbred WKY

Male

Prazosin - analogs & derivatives

RNA, Messenger - metabolism

Azides

Clonidine - analogs & derivatives

Microcirculation

Animals

Renal Circulation

Clonidine - pharmacology

Radioligand Assay

Binding Sites - drug effects

Receptors, Adrenergic, alpha - genetics

Details

Title: Subtitle: Alpha 1B-adrenergic receptors in rat renal microvessels
Creators: Leonardo M Canessa - Department of Pediatrics, Georgetown University Children's Medical Center, Washington, D.C., USA
Marlene M Piccio
Prayong Vachvanichsanong
Anita Sidhu
Craig C Porter
Jean E Robillard
Robin A Felder
Pedro A Jose
Resource Type: Journal article
Publication Details: Kidney international, Vol.48(5), pp.1412-1419
DOI: 10.1038/ki.1995.430
PMID: 8544397
NLM abbreviation: Kidney Int
ISSN: 0085-2538
eISSN: 1523-1755
Publisher: United States
Grant note: DK43961 / NIDDK NIH HHS DK39308 / NIDDK NIH HHS HL23081 / NHLBI NIH HHS
Language: English
Date published: 11/1995
Academic Unit: Molecular Physiology and Biophysics; Nephrology, Dialysis and Transplantation; Stead Family Department of Pediatrics; Medicine Administration
Record Identifier: 9984025598202771

Metrics

29 Record Views

8 Times Cited - Web of Science