Journal article
Alteration in angiogenic and anti-angiogenic forms of vascular endothelial growth factor-A in skeletal muscle of patients with intermittent claudication following exercise training
Vascular medicine (London, England), Vol.17(2), pp.94-100
04/2012
DOI: 10.1177/1358863X11436334
PMCID: PMC3609421
PMID: 22402934
Abstract
The aims of this study were twofold: (1) to identify whether peripheral artery disease (PAD) patients had increased muscle concentration of angiogenic VEGF-A, anti-angiogenic VEGF
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b or VEGF receptor 1 (VEGF-R1) when compared with control subjects, and (2) to evaluate whether exercise training in PAD patients was associated with changes in muscle concentration of VEGF-A, VEGF
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b or VEGF-R1. At baseline, 22 PAD and 30 control subjects underwent gastrocnemius muscle biopsy. Twelve PAD patients were treated with supervised exercise training (SET) and underwent muscle biopsy after 3 weeks and 12 weeks of training and had sufficient tissue to measure VEGF-A, VEGF
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b and VEGF-R1 concentrations in skeletal muscle lysates by ELISA. Muscle concentrations of VEGF-A and VEGF
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b were similar in PAD patients versus controls at baseline. At both time points after the start of SET, VEGF-A levels decreased and there was a trend towards increased VEGF
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b concentrations. At baseline, VEGF-R1 concentrations were lower in PAD patients when compared with controls but did not change after SET. Skeletal muscle concentrations of VEGF-A are not different in PAD patients when compared with controls at baseline. SET is associated with a significant reduction in VEGF-A levels and a trend towards increased VEGF
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b levels. These somewhat unexpected findings suggest that further investigation into the mechanism of vascular responses to exercise training in PAD patients is warranted.
Details
- Title: Subtitle
- Alteration in angiogenic and anti-angiogenic forms of vascular endothelial growth factor-A in skeletal muscle of patients with intermittent claudication following exercise training
- Creators
- W Schuyler Jones - Duke UniversityBrian D Duscha - Duke UniversityJennifer L Robbins - Duke UniversityNatasha N Duggan - University of VirginiaJudith G Regensteiner - University of DenverWilliam E Kraus - Duke UniversityWilliam R Hiatt - University of DenverAyotunde O Dokun - University of VirginiaBrian H Annex - University of Virginia
- Resource Type
- Journal article
- Publication Details
- Vascular medicine (London, England), Vol.17(2), pp.94-100
- DOI
- 10.1177/1358863X11436334
- PMID
- 22402934
- PMCID
- PMC3609421
- ISSN
- 1358-863X
- eISSN
- 1477-0377
- Language
- English
- Date published
- 04/2012
- Academic Unit
- Molecular Physiology and Biophysics; Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984297492102771
Metrics
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