Journal article
Alteration of the DCC tumor-suppressor gene in tumorigenic HPV-18 immortalized human keratinocytes transformed by nitrosomethylurea
Oncogene, Vol.8(1), pp.95-99
01/1993
PMID: 8380923
Abstract
A human papillomavirus type 18 (HPV-18)-immortalized human keratinocyte cell line (1811) has been transformed to tumorigenicity in nude mice by treatment with the carcinogen nitrosomethylurea (NMU). The NMU transformants (1811-NMU-T) showed additional chromosome alterations as compared with parental 1811 cells, including 18q deletion in two of two 1811-NMU-T lines analysed. Restriction fragment length polymorphism (RFLP) analysis indicated that both 1811-NMU-T lines had lost one allele of the 18q deleted in colon cancer (DCC) tumor-suppressor gene. Reverse transcriptase polymerase chain reaction (RT-PCR) showed that DCC expression was absent or barely detectable in the 1811-NMU-T cells as compared with 1811 or normal keratinocytes, suggesting that the remaining DCC allele in the 1811-NMU-T cells was also altered. These studies indicate that reduction or loss of DCC expression may be an important step in NMU transformation of HPV-immortalized cells to tumorigenicity.
Details
- Title: Subtitle
- Alteration of the DCC tumor-suppressor gene in tumorigenic HPV-18 immortalized human keratinocytes transformed by nitrosomethylurea
- Creators
- A J Klingelhutz - Division of Cancer Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104P P SmithL R GarrettJ K McDougall
- Resource Type
- Journal article
- Publication Details
- Oncogene, Vol.8(1), pp.95-99
- Publisher
- England
- PMID
- 8380923
- ISSN
- 0950-9232
- eISSN
- 1476-5594
- Language
- English
- Date published
- 01/1993
- Academic Unit
- Microbiology and Immunology; Radiation Oncology
- Record Identifier
- 9984002384402771
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