Journal article
Alterations in heat-induced radiosensitization accompanied by nuclear structure alterations in Chinese hamster cells
International journal of hyperthermia, Vol.22(1), pp.43-60
02/2006
DOI: 10.1080/02656730500394296
PMID: 16423752
Abstract
This paper examined heat-induced radiosensitization in two Chinese hamster heat-resistant cell lines, HR-1 and OC-14, that were isolated from the same wild-type HA-1 cell line. It found a reduction of the magnitude of heat-induced radiosensitization after exposure to 43 degrees C in both HR-1 and OC-14 cells and a similar reduction after exposure to 45 degrees C in HR-1 cells, but not in OC-14 cells. The effect of heat exposure on a class of ionizing radiation-induced DNA damage that inhibits the ability of nuclear DNA to undergo super-coiling changes was also studied using the fluorescent halo assay in these three cell lines. Wild type cells exposed to either 43 or 45 degrees C before irradiation had a DNA rewinding ability that was intermediate between control and unheated cells, a phenomenon previously described as a masking effect. This masking effect was significantly reduced in HR-1 cells exposed to either 43 or 45 degrees C or in OC-14 cells exposed to 43 degrees C under conditions that heat-induced radiosensitization was reduced. In contrast, the masking effect was not altered in OC-14 cells exposed to 45 degrees C, conditions under which heat-induced radiosensitization was similar to that observed in wild-type HA-1 cells. These results suggest that a reduction in the masking effect is associated with a reduction of the magnitude of heat-induced radiosensitization in the HR-1 and OC-14 heat-resistant cell lines. The reduction of the masking effect in the cell lines resistant to heat-induced radiosensitization was associated with neither a reduction in the magnitude of the heat-induced increase in total nuclear protein content nor major differences in the protein profiles of the nucleoids isolated from heated cells.
Details
- Title: Subtitle
- Alterations in heat-induced radiosensitization accompanied by nuclear structure alterations in Chinese hamster cells
- Creators
- Andrei Laszlo - Washington University in St. LouisTeri Davidson - Washington University in St. LouisAmanda Harvey - Washington University in St. LouisJulia E Sim - Washington University in St. LouisRobert S Malyapa - Washington University in St. LouisDouglas R Spitz - University of IowaJoseph L Roti Roti
- Resource Type
- Journal article
- Publication Details
- International journal of hyperthermia, Vol.22(1), pp.43-60
- DOI
- 10.1080/02656730500394296
- PMID
- 16423752
- NLM abbreviation
- Int J Hyperthermia
- ISSN
- 0265-6736
- eISSN
- 1464-5157
- Grant note
- P01-CA-75556 / NCI NIH HHS HL-51469 / NHLBI NIH HHS
- Language
- English
- Date published
- 02/2006
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984313092102771
Metrics
27 Record Views