Journal article
Alterations in the muscle force transfer apparatus in aged rats during unloading and reloading: impact of microRNA‐31
The Journal of physiology, Vol.596(14), pp.2883-2900
07/14/2018
DOI: 10.1113/JP275833
PMCID: PMC6046073
PMID: 29726007
Abstract
Force transfer is integral for maintaining skeletal muscle structure and function. One important component is dystrophin. There is limited understanding of how force transfer is impacted by age and loading.
Here, we investigate the force transfer apparatus in muscles of adult and old rats exposed to periods of disuse and reloading.
Our results demonstrate an increase in dystrophin protein during the reloading phase in the adult tibialis anterior muscle that is delayed in the old muscle. The consequence of this delay is an increased susceptibility towards contraction‐induced muscle injury.
Central to the lack of dystrophin protein is an increase in miR‐31, a microRNA that inhibits dystrophin translation. In vivo electroporation with a miR‐31 sponge led to increased dystrophin protein and decreased contraction‐induced muscle injury in old skeletal muscle.
Overall, our results detail the importance of the force transfer apparatus and provide new mechanisms for contraction‐induced injury in ageing skeletal muscle.
Details
- Title: Subtitle
- Alterations in the muscle force transfer apparatus in aged rats during unloading and reloading: impact of microRNA‐31
- Creators
- David C. Hughes - University of California, DavisGeorge R. Marcotte - University of California, DavisLeslie M. Baehr - University of California, DavisDaniel W. D. West - University of California, DavisAndrea G. Marshall - University of California, DavisScott M. Ebert - University of IowaArik Davidyan - University of California, DavisChristopher M. Adams - University of IowaSue C Bodine - University of California, DavisKeith Baar - University of California, Davis
- Resource Type
- Journal article
- Publication Details
- The Journal of physiology, Vol.596(14), pp.2883-2900
- DOI
- 10.1113/JP275833
- PMID
- 29726007
- PMCID
- PMC6046073
- NLM abbreviation
- J Physiol
- ISSN
- 0022-3751
- eISSN
- 1469-7793
- Publisher
- John Wiley and Sons Inc
- Grant note
- R01AG045375 / HHS | NIH | National Institute on Aging (U.S. National Institute on Aging) VARR&D E7766‐R / Center for Integrated Healthcare, U.S. Department of Veterans Affairs (VISN 2 Center for Integrated Healthcare)
- Alternative title
- D. C. Hughes and others
- Language
- English
- Date published
- 07/14/2018
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359828502771
Metrics
16 Record Views