Alterations of long noncoding RNAs and mRNAs in extracellular vesicles derived from the murine heart post-ischemia-reperfusion injury

Xinyu Ge; Qingshu Meng; Xuan Liu; Jing Liu; Xiaoxue Ma; Shanshan Shi; Mimi Li; Fang Lin; Xiaoting Liang; Xin Gong; Zhongmin Liu; Wei Han; Xiaohui Zhou

doi:10.1111/jcmm.17617

Back

Alterations of long noncoding RNAs and mRNAs in extracellular vesicles derived from the murine heart post-ischemia-reperfusion injury

Journal article

Open access

Peer reviewed

Alterations of long noncoding RNAs and mRNAs in extracellular vesicles derived from the murine heart post-ischemia-reperfusion injury

Xinyu Ge, Qingshu Meng, Xuan Liu, Jing Liu, Xiaoxue Ma, Shanshan Shi, Mimi Li, Fang Lin, Xiaoting Liang, Xin Gong, …

Journal of cellular and molecular medicine, Vol.26(24), pp.6006-6018

11/28/2022

DOI: 10.1111/jcmm.17617

PMCID: PMC9753460

PMID: 36444487

Files and links (1)

url

https://doi.org/10.1111/jcmm.17617View

Published (Version of record) Open Access

Abstract

Extracellular vesicles (EVs) play important roles in cardiovascular diseases by delivering their RNA cargos. However, the features and possible role of the lncRNAs and mRNAs in cardiac EVs during ischemia-reperfusion (IR) remain unclear. Therefore, we performed RNA sequencing analysis to profile the features of lncRNAs and mRNAs and predicted their potential functions. Here, we demonstrated that the severity of IR injury was significantly correlated with cardiac EV production. RNA sequencing identified 73 significantly differentially expressed (DE) lncRNAs (39 upregulated and 34 downregulated) and 720 DE-mRNAs (317 upregulated and 403 downregulated). Gene Ontology (GO) and pathway analysis were performed to predict the potential functions of the DE-lncRNAs and mRNAs. The lncRNA-miRNA-mRNA ceRNA network showed the possible functions of DE-lncRNAs with DE-mRNAs which are enriched in the pathways of T cell receptor signalling pathway and cell adhesion molecules. Moreover, the expressions of ENSMUST00000146010 and ENSMUST00000180630 were negatively correlated with the severity of IR injury. A significant positive correlation was revealed between TCONS_00010866 expression and the severity of the cardiac injury. These findings revealed the lncRNA and mRNA profiles in the heart derived EVs and provided potential targets and pathways involved in cardiac IR injury.

Details

Title: Subtitle: Alterations of long noncoding RNAs and mRNAs in extracellular vesicles derived from the murine heart post-ischemia-reperfusion injury
Creators: Xinyu Ge
Qingshu Meng
Xuan Liu
Jing Liu
Xiaoxue Ma
Shanshan Shi
Mimi Li
Fang Lin
Xiaoting Liang
Xin Gong
Zhongmin Liu
Wei Han
Xiaohui Zhou
Resource Type: Journal article
Publication Details: Journal of cellular and molecular medicine, Vol.26(24), pp.6006-6018
DOI: 10.1111/jcmm.17617
PMID: 36444487
PMCID: PMC9753460
NLM abbreviation: J Cell Mol Med
ISSN: 1582-1838
eISSN: 1582-4934
Grant note: DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 81670458, 82100350
Language: English
Date published: 11/28/2022
Academic Unit: Anatomy and Cell Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984322741602771

Metrics

17 Record Views

3 Times Cited - Web of Science