Journal article
Alterations of the Portal Protein, gpB, of Bacteriophage λ Suppress Mutations in cosQ , the Site Required for Termination of DNA Packaging
Genetics (Austin), Vol.161(1), pp.21-31
05/01/2002
DOI: 10.1093/genetics/161.1.21
PMCID: PMC1462103
PMID: 12019220
Abstract
Abstract
The cosQ site of bacteriophage λ is required for DNA packaging termination. Previous studies have shown that cosQ mutations can be suppressed in three ways: by a local suppressor within cosQ, an increase in the length of the λ chromosome, and missense mutations affecting the prohead’s portal protein, gpB. In the present work, revertants of a set of lethal cosQ mutants were screened for suppressors. Seven new cosQ suppressors affected gene B, which encodes the portal protein of the prohead. All seven were allelenonspecific suppressors of cosQ mutations. Experiments with several phages having two cosQ suppressors showed that the suppression effects were additive. Furthermore, these double suppressors had minimal effects on the growth of cosQ+ phages. These trans-acting suppressors affecting the portal protein are proposed to allow the mutant cosQ site to be more efficiently recognized, due to the slowing of the rate of translocation.
Details
- Title: Subtitle
- Alterations of the Portal Protein, gpB, of Bacteriophage λ Suppress Mutations in cosQ , the Site Required for Termination of DNA Packaging
- Creators
- Douglas J Wieczorek - University of IowaLisa Didion - University of IowaMichael Feiss - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.161(1), pp.21-31
- DOI
- 10.1093/genetics/161.1.21
- PMID
- 12019220
- PMCID
- PMC1462103
- ISSN
- 1943-2631
- eISSN
- 1943-2631
- Language
- English
- Date published
- 05/01/2002
- Academic Unit
- Microbiology and Immunology; Teaching and Learning
- Record Identifier
- 9984383908002771
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