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Altered IFN-γ and IL-4 pattern lymphokine secretion in mice partially depleted of CD4 T cells by anti-CD4 monoclonal antibody
Journal article   Peer reviewed

Altered IFN-γ and IL-4 pattern lymphokine secretion in mice partially depleted of CD4 T cells by anti-CD4 monoclonal antibody

Elizabeth H Field, Todd M Rouse, Audrey L Fleming, Iyad Jamali and John S Cowdery
The Journal of immunology (1950), Vol.149(4), pp.1131-1137
1992
DOI: 10.4049/jimmunol.149.4.1131
PMID: 1354229

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Abstract

Complete elimination of CD4 cells by in vivo treatment with anti-CD4 mAb may result in B cell polyclonal activation. Additionally, mice treated with doses of anti-CD4 that eliminate half the CD4 cells produced higher anti-SRBC antibody responses than controls. This suggests that partial CD4 depletion enhances Th2-like function. To test this hypothesis we examined Th1 and Th2 lymphokine potential in mice partially depleted of CD4 cells. We measured IL-4 and IFN-gamma secretion by stimulated unfractionated spleen cells and analyzed activated, purified CD4 cells by RNA in situ hybridization to determine the percentage of IFN-gamma- or IL-4-producing cells. Unfractionated splenocytes from partially CD4-depleted mice secreted more IL-4 and less IFN-gamma than splenocytes from control mice. In situ hybridization proved that CD4 cells from partially depleted mice contained a higher percentage of IL-4 and a lower percentage of IFN-gamma-producing cells than controls. These results indicate that treatment with a dose of mAb resulting in partial CD4 depletion may permit increased Th2-like lymphokine expression. This study also provides evidence that cells committed to Th2-like function exist in vivo in mice.
 Immunobiology Fundamental and applied biological sciences. Psychology Fundamental immunology Biological and medical sciences Regulatory factors and their cellular receptors Analysis of the immune response. Humoral and cellular immunity Lymphokines, interleukins ( function, expression)

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