Altered bone-regulating myokine expression in skeletal muscle Of Duchenne muscular dystrophy mouse models

Shumin Zhou; Baoli Qian; Ling Wang; Changqing Zhang; Macalus V Hogan; Hongshuai Li

doi:10.1002/mus.26195

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Altered bone-regulating myokine expression in skeletal muscle Of Duchenne muscular dystrophy mouse models

Journal article

Peer reviewed

Altered bone-regulating myokine expression in skeletal muscle Of Duchenne muscular dystrophy mouse models

Shumin Zhou, Baoli Qian, Ling Wang, Changqing Zhang, Macalus V Hogan and Hongshuai Li

Muscle & nerve, Vol.58(4), pp.573-582

10/2018

DOI: 10.1002/mus.26195

PMID: 30028902

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Abstract

Duchenne muscular dystrophy (DMD) has been well characterized as a disease that affects both skeletal muscle and bone. The pathophysiology responsible for the deficits in bone tissue is still unclear. Quantitative reverse-transcription polymerase chain reaction and Western blot analyses of known myokines from skeletal muscle were performed on dystrophic mouse models and wild-type (WT) controls to identify differentially expressed bone-regulating myokines. Twenty-four of 43 myokine genes demonstrated significantly different mRNA expression in the skeletal muscles of dystrophic mice when compared with muscles of WT mice. Several differently expressed bone-regulating myokine genes were identified, and their protein levels were also verified by Western blot. Dystrophic skeletal muscle demonstrated a significantly altered myokine gene expression profile. mRNA and protein levels of several bone-regulating myokines were significantly altered in dystrophic skeletal muscle, which suggests pathological role of bone-regulating myokines on bone homeostasis in DMD. Muscle Nerve 58: 573-582, 2018.

Cell Adhesion Molecules - genetics

RANK Ligand - metabolism

Fibroblast Growth Factors - genetics

Muscle, Skeletal - metabolism

Leukemia Inhibitory Factor - genetics

Femur - diagnostic imaging

RNA, Messenger - metabolism

X-Ray Microtomography

Case-Control Studies

Leukemia Inhibitory Factor - metabolism

Fibroblast Growth Factors - metabolism

Osteonectin - genetics

Bone and Bones - diagnostic imaging

Bone and Bones - metabolism

Mice, Inbred mdx

Myostatin - genetics

Myostatin - metabolism

Cytokines - genetics

Interleukin-6 - metabolism

Disease Models, Animal

Cytokines - metabolism

Interleukin-6 - genetics

Lumbar Vertebrae - diagnostic imaging

Osteonectin - metabolism

Reverse Transcriptase Polymerase Chain Reaction

Cell Adhesion Molecules - metabolism

Blotting, Western

Fibronectins - metabolism

Bone Diseases, Metabolic - metabolism

RANK Ligand - genetics

Animals

Lower Extremity

Fibronectins - genetics

Mice

Muscular Dystrophy, Duchenne - metabolism

Bone Diseases, Metabolic - genetics

Muscular Dystrophy, Duchenne - genetics

Details

Title: Subtitle: Altered bone-regulating myokine expression in skeletal muscle Of Duchenne muscular dystrophy mouse models
Creators: Shumin Zhou - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China
Baoli Qian - Musculoskeletal Growth & Regeneration Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, 15219, USA
Ling Wang - Vascular Medicine Institute, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
Changqing Zhang - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China
Macalus V Hogan - Musculoskeletal Growth & Regeneration Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, 15219, USA
Hongshuai Li - Musculoskeletal Growth & Regeneration Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, 15219, USA
Resource Type: Journal article
Publication Details: Muscle & nerve, Vol.58(4), pp.573-582
DOI: 10.1002/mus.26195
PMID: 30028902
ISSN: 0148-639X
eISSN: 1097-4598
Grant note: DOI: 10.13039/100000945, name: Pittsburgh Foundation, award: AD2016‐1765‐29
Language: English
Date published: 10/2018
Academic Unit: Orthopedics and Rehabilitation; Radiation Oncology
Record Identifier: 9984106393402771

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