Journal article
Altered cytokine production and accessory cell function after HIV-1 infection
The Journal of immunology (1950), Vol.157(3), pp.1313-1320
08/01/1996
DOI: 10.4049/jimmunol.157.3.1313
PMID: 8757640
Abstract
We investigated cytokine production and accessory cell function in human macrophage hybridoma cell lines and primary monocytes after infection with HIV-1. HIV-1 infection induced IL-10 production in the macrophage hybridoma cell line with loss of IL-12 1 wk after infection. There were also significant increases in production of IL-10 (537 +/- 521 vs 687 +/- 625 pg/ml) while there was a reduction in IL-12 (6.3 +/- 3.1 vs 1.2 +/- 1.0 pg/ml, p = 0.021) in the primary monocytes 5 days after HIV-1 infection. In addition, the hybridoma cell lines and primary monocytes failed to support PHA, Con A, PWM, or anti-CD3- induced T cell proliferation 1 wk after infection. The viability of the T cells cocultured with the HIV-1-infected macrophage cell lines or the primary monocytes as determined by propidium iodide staining was unaltered and there was no increase in apoptosis-specific DNA strand breaks or increased expression of Bcl-2 in the T cells. No soluble suppressor factor was present, since UV-inactivated supernatants from the hybridoma cell line and primary monocytes failed to inhibit mitogen- and anti-CD3-induced T cell proliferation. Early events in T cell activation, including calcium flux and phosphotyrosine kinase activity, were intact in the T cells cocultured with the HIV-1- infected hybridomas and monocytes but there was reduced IL-2 production. Addition of exogenous IL-2 restored the proliferative responses. Taken together, these data suggest that alteration of cytokine production and accessory cell function for mitogens and anti-CD3-induced T cell proliferation independent of induction of apoptosis, suppressor factor production, or inhibition of T cell signaling occurs very early after HIV-1 infection and may contribute to the global immunosuppression observed in AIDS.
Details
- Title: Subtitle
- Altered cytokine production and accessory cell function after HIV-1 infection
- Creators
- James Yoo - Division of Clinical Immunology, Mount Sinai Medical Center, New York, NY 10029, USAHouchu ChenThomas KrausDavid HirschSteven PolyakItalas GeorgeKirk Sperber
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.157(3), pp.1313-1320
- DOI
- 10.4049/jimmunol.157.3.1313
- PMID
- 8757640
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- T35 AM07420 / NIADDK NIH HHS CA R29 256990 / NCI NIH HHS
- Language
- English
- Date published
- 08/01/1996
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984094714802771
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