Altered fluid transport across airway epithelium in cystic fibrosis

CANWEN JIANG; W. E FINKBEINER; J. H WIDDICOMBE; P. B MCCRAY; S. S MILLER

doi:10.1126/science.8211164

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Altered fluid transport across airway epithelium in cystic fibrosis

Journal article

Peer reviewed

Altered fluid transport across airway epithelium in cystic fibrosis

CANWEN JIANG, W. E FINKBEINER, J. H WIDDICOMBE, P. B MCCRAY and S. S MILLER

Science (American Association for the Advancement of Science), Vol.262(5132), pp.424-427

1993

DOI: 10.1126/science.8211164

PMID: 8211164

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Abstract

In cystic fibrosis (CF), absence or dysfunction of a phosphorylation-regulated chloride channel [CF transmembrane conductance regulator (CFTR)] leads to the loss or reduction of chloride secretion into the airways. Active sodium absorption is also increased in CF, and both of these ion transport changes could alter fluid transport across the airways. Under baseline conditions, cultured human airway epithelia from normal individuals absorbed fluid, and this absorption was increased in epithelia from patients with CF. In normal and CF epithelial cultures fluid absorption was inhibited by amiloride. Adenosine 3',5'-monophosphate stimulated fluid secretion in normal epithelial cultures but not in cultures from individuals with CF. In contrast, fluid secretion induced by nucleotide triphosphates (uridine triphosphate or adenosine triphosphate) was unaltered in cultures of epithelia from patients with CF, suggesting an approach to the treatment of CF.

Gastroenterology. Liver. Pancreas. Abdomen

Biological and medical sciences

Medical sciences

Liver. Biliary tract. Portal circulation. Exocrine pancreas

Other diseases. Semiology

Details

Title: Subtitle: Altered fluid transport across airway epithelium in cystic fibrosis
Creators: CANWEN JIANG - Univ. California, school optometry, div. cell development, Berkeley CA 94720, United States
W. E FINKBEINER - Univ. California, school optometry, div. cell development, Berkeley CA 94720, United States
J. H WIDDICOMBE - Univ. California, school optometry, div. cell development, Berkeley CA 94720, United States
P. B MCCRAY - Univ. California, school optometry, div. cell development, Berkeley CA 94720, United States
S. S MILLER - Univ. California, school optometry, div. cell development, Berkeley CA 94720, United States
Resource Type: Journal article
Publication Details: Science (American Association for the Advancement of Science), Vol.262(5132), pp.424-427
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.8211164
PMID: 8211164
ISSN: 0036-8075
eISSN: 1095-9203
Language: English
Date published: 1993
Academic Unit: Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
Record Identifier: 9984093458302771

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