Altered surfactant protein A gene expression and protein metabolism associated with repeat exposure to inhaled endotoxin

Caroline L S George; Misty L White; Marsha E O'Neill; Peter S Thorne; David A Schwartz; Jeanne M Snyder

doi:10.1152/ajplung.00064.2003

Back

Altered surfactant protein A gene expression and protein metabolism associated with repeat exposure to inhaled endotoxin

Journal article

Open access

Peer reviewed

Altered surfactant protein A gene expression and protein metabolism associated with repeat exposure to inhaled endotoxin

Caroline L S George, Misty L White, Marsha E O'Neill, Peter S Thorne, David A Schwartz and Jeanne M Snyder

American journal of physiology. Lung cellular and molecular physiology, Vol.285(6), pp.L1337-1344

12/2003

DOI: 10.1152/ajplung.00064.2003

PMID: 12922979

Files and links (1)

url

https://doi.org/10.1152/ajplung.00064.2003View

Published (Version of record) Open Access

Abstract

Chronically inhaled endotoxin, which is ubiquitous in many occupational and domestic environments, can adversely affect the respiratory system resulting in an inflammatory response and decreased lung function. Surfactant-associated protein A (SP-A) is part of the lung innate immune system and may attenuate the inflammatory response in various types of lung injury. Using a murine model to mimic occupational exposures to endotoxin, we hypothesized that SP-A gene expression and protein would be elevated in response to repeat exposure to inhaled grain dust and to purified lipopolysaccharide (LPS). Our results demonstrate that repeat exposure to inhaled endotoxin, either in the form of grain dust or purified LPS, results in increased whole lung SP-A gene expression and type II alveolar epithelial cell hyperplasia, whereas SP-A protein levels in lung lavage fluid are decreased. Furthermore, these alterations in SP-A gene activity and protein metabolism are dependent on an intact endotoxin signaling system.

Bronchoalveolar Lavage Fluid

Pneumonia - physiopathology

Signal Transduction

Occupational Exposure

Pulmonary Surfactant-Associated Protein A - metabolism

Mice, Inbred C3H

Membrane Glycoproteins - genetics

Toll-Like Receptors

Dust

Animals

Mice, Mutant Strains

Gene Expression - physiology

Lipopolysaccharides - pharmacology

Edible Grain

Mice

Respiratory Mucosa - metabolism

Pneumonia - metabolism

Chronic Disease

Pulmonary Surfactant-Associated Protein A - genetics

Disease Models, Animal

Receptors, Cell Surface - genetics

Details

Title: Subtitle: Altered surfactant protein A gene expression and protein metabolism associated with repeat exposure to inhaled endotoxin
Creators: Caroline L S George - Department of Pediatrics, Division of Pediatric Critical Care, University of Iowa, Iowa City, IA 52242, USA. caroline-george@uiowa.edu
Misty L White
Marsha E O'Neill
Peter S Thorne
David A Schwartz
Jeanne M Snyder
Resource Type: Journal article
Publication Details: American journal of physiology. Lung cellular and molecular physiology, Vol.285(6), pp.L1337-1344
DOI: 10.1152/ajplung.00064.2003
PMID: 12922979
NLM abbreviation: Am J Physiol Lung Cell Mol Physiol
ISSN: 1040-0605
eISSN: 1522-1504
Publisher: United States
Grant note: HD-27748 / NICHD NIH HHS ES-07498 / NIEHS NIH HHS ES-09607 / NIEHS NIH HHS
Language: English
Date published: 12/2003
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health; Anatomy and Cell Biology; Stead Family Department of Pediatrics
Record Identifier: 9983997370502771

Metrics

42 Record Views

20 Times Cited - Web of Science