Journal article
Alveolar Airspace Size in Healthy and Diseased Infant Lungs Measured via Hyperpolarized He-3 Gas Diffusion Magnetic Resonance Imaging
Neonatology (Basel, Switzerland), Vol.117(6), pp.704-712
02/01/2021
DOI: 10.1159/000511084
PMCID: PMC7878286
PMID: 33176330
Abstract
Background: Alveolar development and lung parenchymal simplification are not well characterized in vivo in neonatal patients with respiratory morbidities, such as bronchopulmonary dysplasia (BPD). Hyperpolarized (HP) gas diffusion magnetic resonance imaging (MRI) is a sensitive, safe, nonionizing, and noninvasive biomarker for measuring airspace size in vivo but has not yet been implemented in young infants. Objective: This work quantified alveolar airspace size via HP gas diffusion MRI in healthy and diseased explanted infant lung specimens, with comparison to histological morphometry. Methods: Lung specimens from 8 infants were obtained: 7 healthy left upper lobes (0-16 months, post-autopsy) and 1 left lung with filamin-A mutation, closely representing BPD lung disease (11 months, post-transplantation). Specimens were imaged using HP He-3 diffusion MRI to generate apparent diffusion coefficients (ADCs) as biomarkers of alveolar airspace size, with comparison to mean linear intercept (L-m) via quantitative histology. Results: Mean ADC and L-m were significantly increased throughout the diseased specimen (ADC = 0.26 +/- 0.06 cm(2)/s, L-m = 587 +/- 212 mu m) compared with healthy specimens (ADC = 0.14 +/- 0.03 cm(2)/s, L-m = 133 +/- 37 mu m; p < 1 x 10(-7)); increased values reflect enlarged airspaces. Mean ADCs in healthy specimens were significantly correlated to L-m (r = 0.69, p = 0.041). Conclusions: HP gas diffusion MRI is sensitive to healthy and diseased regional alveolar airspace size in infant lungs, with good comparison to quantitative histology in ex vivo specimens. This work demonstrates the translational potential of gas MRI techniques for in vivo assessment of normal and abnormal alveolar development in neonates with pulmonary disease.
Details
- Title: Subtitle
- Alveolar Airspace Size in Healthy and Diseased Infant Lungs Measured via Hyperpolarized He-3 Gas Diffusion Magnetic Resonance Imaging
- Creators
- Nara S. Higano - Cincinnati Children's Hospital Medical CenterRobert P. Thomen - University of MissouriJames D. Quirk - Washington University in St. LouisHeidie L. Huyck - University of RochesterAndrew D. Hahn - University of Wisconsin–MadisonSean B. Fain - University of Wisconsin–MadisonGloria S. Pryhuber - University of RochesterJason C. Woods - Cincinnati Children's Hospital Medical Center
- Resource Type
- Journal article
- Publication Details
- Neonatology (Basel, Switzerland), Vol.117(6), pp.704-712
- DOI
- 10.1159/000511084
- PMID
- 33176330
- PMCID
- PMC7878286
- NLM abbreviation
- Neonatology
- ISSN
- 1661-7800
- eISSN
- 1661-7819
- Publisher
- Karger
- Number of pages
- 9
- Grant note
- LungMAP University of Wisconsin Department of Radiology Research and Development Fund U01 HL122700; P01 HL070831; T32 HL007752; T32 CA009206 / National Institutes of Health: NHLBI Biorepository United Network for Organ Sharing U01 HL122642 / National Institutes of Health: NHLBI LungMAP Consortium Research Foundation at Cincinnati Children's Hospital Hartwell Foundation Perinatal Institute
- Language
- English
- Date published
- 02/01/2021
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Health, Sport, and Human Physiology
- Record Identifier
- 9984274953102771
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