Alveolarization in retinoic acid receptor-beta-deficient mice

Jeanne M Snyder; Melinda Jenkins-Moore; Sheila K Jackson; Kelli L Goss; Hui-Hui Dai; Peter J Bangsund; Vincent Giguere; Stephen E McGowan

doi:10.1203/01.PDR.0000151315.81106.D3

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Alveolarization in retinoic acid receptor-beta-deficient mice

Journal article

Open access

Peer reviewed

Alveolarization in retinoic acid receptor-beta-deficient mice

Jeanne M Snyder, Melinda Jenkins-Moore, Sheila K Jackson, Kelli L Goss, Hui-Hui Dai, Peter J Bangsund, Vincent Giguere and Stephen E McGowan

Pediatric research, Vol.57(3), pp.384-391

03/2005

DOI: 10.1203/01.PDR.0000151315.81106.D3

PMID: 15635054

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Abstract

Retinoids bind to nuclear receptors [retinoic acid receptors (RARs) and retinoid X receptors]. RARbeta, one of three isoforms of RARs (alpha, beta, and gamma), is expressed in the fetal and adult lung. We hypothesized that RARbeta plays a role in alveolarization. Using morphometric analysis, we determined that there was a significant increase in the volume density of airspace in the alveolar region of the lung at 28, 42, and 56 d postnatal age in RARbeta null mice when compared with wild-type controls. The mean cord length of the respiratory airspaces was increased in RARbeta null animals at 42 d postnatal age. Respiratory gas-exchange surface area per unit lung volume was significantly decreased in RARbeta null animals at 28, 42, and 56 d postnatal age. In addition, alveolar ducts tended to comprise a greater proportion of the lung airspaces in the RARbeta null mice. The RARbeta null mice also had impaired respiratory function when compared with wild-type control mice. There was no effect of RARbeta gene deletion on lung platelet-derived growth factor (PDGF) receptor alpha mRNA levels in postnatal lung tissue at several postnatal ages. However PDGF-A protein levels were significantly lower in the RARbeta null mice than in wild-type controls. Thus, deletion of the RARbeta gene impairs the formation of the distal airspaces during the postnatal phase of lung maturation in mice via a pathway that may involve PDGF-A.

Pulmonary Alveoli - growth & development

Retinoids - metabolism

Mice, Inbred C57BL

Pulmonary Alveoli - physiology

Male

Receptors, Retinoic Acid - metabolism

Random Allocation

Platelet-Derived Growth Factor - genetics

Receptors, Retinoic Acid - genetics

Mice, Knockout

Platelet-Derived Growth Factor - metabolism

Animals

Protein Isoforms - metabolism

Female

Mice

Pulmonary Alveoli - anatomy & histology

Protein Isoforms - genetics

Details

Title: Subtitle: Alveolarization in retinoic acid receptor-beta-deficient mice
Creators: Jeanne M Snyder - Department of Anatomy, University of Iowa College of Medicine, Iowa City, IA 52242, USA. jeanne-snyder@uiowa.edu
Melinda Jenkins-Moore
Sheila K Jackson
Kelli L Goss
Hui-Hui Dai
Peter J Bangsund
Vincent Giguere
Stephen E McGowan
Resource Type: Journal article
Publication Details: Pediatric research, Vol.57(3), pp.384-391
DOI: 10.1203/01.PDR.0000151315.81106.D3
PMID: 15635054
NLM abbreviation: Pediatr Res
ISSN: 0031-3998
eISSN: 1530-0447
Publisher: United States
Grant note: HL53430 / NHLBI NIH HHS HL62861 / NHLBI NIH HHS
Language: English
Date published: 03/2005
Academic Unit: Anatomy and Cell Biology; International Programs; Stead Family Department of Pediatrics; Internal Medicine
Record Identifier: 9983984524602771

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