Ameliorative potential of aliskiren in experimental colitis in mice

Rakesh B Patel; Kanaiyalal D Prajapati; Bhavin M Sonara; Manoranjan M Sharma; Hiren M Patel; Vishwanath D Pawar; Mukul R Jain

doi:10.1016/j.ejphar.2014.05.009

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Ameliorative potential of aliskiren in experimental colitis in mice

Journal article

Peer reviewed

Ameliorative potential of aliskiren in experimental colitis in mice

Rakesh B Patel, Kanaiyalal D Prajapati, Bhavin M Sonara, Manoranjan M Sharma, Hiren M Patel, Vishwanath D Pawar and Mukul R Jain

European journal of pharmacology, Vol.737, pp.70-76

08/15/2014

DOI: 10.1016/j.ejphar.2014.05.009

PMID: 24858618

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Abstract

In the present study, we investigated the ameliorative potential of aliskiren in dextran sulfate sodium (DSS) induced colitis in mice. Aliskiren (3 and 10mg/kg, i.p.) was administered for 10 days from the day of DSS administration. The severity of colitis in mice was assessed using body weight loss, colon and spleen weight, hematological parameters, food intake, stool consistency, rectal bleeding and colon shortening. Colonic malondialdehyde (MDA), myeloperoxidase (MPO) and renin mRNA levels were also estimated. Furthermore, TNF-α and IL-6 in plasma and colon were analyzed. The results showed that aliskiren (10mg/kg, i.p.) significantly improved the severity of colitis by, decrease in weight loss, improvement in food intake and stool consistency, decrease in rectal bleeding, decrease in relative colon and spleen weight and improvement in colonic shortening. Aliskiren (10mg/kg, i.p.) improved blood hemoglobin, red blood cells (RBC) and hematocrit. Colonic malondialdehyde (MDA), MPO and histolopathological score were significantly diminished by aliskiren (10mg/kg, i.p.). Furthermore, aliskiren (10mg/kg, i.p.) significantly diminished the elevated levels of TNF-α, IL-6 and renin mRNA in inflammed colon. These results indicate involvement of renin in colitis and inhibition of renin by aliskiren ameliorates colitis.

Amides - pharmacology

Amides - therapeutic use

Animals

Anti-Inflammatory Agents - pharmacology

Anti-Inflammatory Agents - therapeutic use

Antioxidants - pharmacology

Antioxidants - therapeutic use

Body Weight - drug effects

Colitis - blood

Colitis - chemically induced

Colitis - drug therapy

Colitis - pathology

Colon - drug effects

Colon - pathology

Cytokines - metabolism

Dextran Sulfate - adverse effects

Eating - drug effects

Erythrocytes - drug effects

Female

Fumarates - pharmacology

Fumarates - therapeutic use

Gene Expression Regulation - drug effects

Hematocrit

Hemoglobins - metabolism

Malondialdehyde - metabolism

Mice

Organ Size - drug effects

Peroxidase - metabolism

Renin - genetics

RNA, Messenger - genetics

RNA, Messenger - metabolism

Spleen - drug effects

Spleen - pathology

Details

Title: Subtitle: Ameliorative potential of aliskiren in experimental colitis in mice
Creators: Rakesh B Patel - Cadila Healthcare
Kanaiyalal D Prajapati - Cadila Healthcare
Bhavin M Sonara - Cadila Healthcare
Manoranjan M Sharma - Cadila Healthcare
Hiren M Patel - Cadila Healthcare
Vishwanath D Pawar - Cadila Healthcare
Mukul R Jain - Cadila Healthcare
Resource Type: Journal article
Publication Details: European journal of pharmacology, Vol.737, pp.70-76
DOI: 10.1016/j.ejphar.2014.05.009
PMID: 24858618
ISSN: 0014-2999
eISSN: 1879-0712
Language: English
Date published: 08/15/2014
Academic Unit: Internal Medicine
Record Identifier: 9985132071702771

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